Gene Expression Data
Assay Details
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Assay
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Reference:
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J:111698
Fish JL, et al., Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells. Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10438-43
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6704895
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Gene symbol:
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Aspm
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Gene name:
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abnormal spindle microtubule assembly
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Antibody:
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Aspm antibody
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
1A:
embryonic day 12.5
(more  )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Specimen
1A:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
1A Aspm
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1A Merge
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Expression is in the dorsal telencephalon and protein is concentrated at the poles of the mitotic spindle in neuroepithelial cells.
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Specimen
1B:
embryonic day 14.5; Btg2tm1Wbh/Btg2 +
(more )
Note: Double labeled: white - Aspm; green - Btg2 reporter (assay MGI:6704898).
Specimen
1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Btg2tm1Wbh/Btg2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: white - Aspm; green - Btg2 reporter (assay MGI:6704898).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
1B Aspm
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Expression is associated with the spindle poles and GFP-negative neuroepithelial cells had stronger staining at spindle poles than did GFP-positive neuroepithelial cells.
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Specimen
6 prometa:
embryonic day 12.5; Btg2tm1Wbh/Btg2 +
(more )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Specimen
6 prometa:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Btg2tm1Wbh/Btg2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
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6_prometa Aspm
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6_prometa merge
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Expression is associated with the spindle poles during all phases of mitosis, with a decrease in the intensity of immunostaining in telophase in neuroepithelial cells.
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Specimen
6 meta:
embryonic day 12.5; Btg2tm1Wbh/Btg2 +
(more )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Specimen
6 meta:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Btg2tm1Wbh/Btg2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
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6_meta merge
,
6_meta Aspm
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Expression is associated with the spindle poles during all phases of mitosis, with a decrease in the intensity of immunostaining in telophase in neuroepithelial cells.
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Specimen
6 telo:
embryonic day 12.5; Btg2tm1Wbh/Btg2 +
(more )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Specimen
6 telo:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Btg2tm1Wbh/Btg2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
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6_telo Aspm
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6_telo merge
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Expression is associated with the spindle poles during all phases of mitosis, with a decrease in the intensity of immunostaining in telophase in neuroepithelial cells.
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