Fap RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:59998 Niedermeyer J, et al., Targeted disruption of mouse fibroblast activation protein. Mol Cell Biol. 2000 Feb;20(3):1089-94
Assay type:	RT-PCR
MGI Accession ID:	MGI:6694014
Gene symbol:	Fap
Gene name:	fibroblast activation protein

Probe:

mFap7, mFap36

Results

Image: 2B Exon3-6

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	300 bp	135 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
-/-	E14.5	TS22: embryo	Absent	Present	25 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Fap^tm1Schn/Fap^tm1Schn	Not Specified
-/-	E14.5	TS22: embryo	Absent	Present	25 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Fap^tm1Schn/Fap^tm1Schn	Not Specified
+/-	E14.5	TS22: embryo	Present	Present	25 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Fap^tm1Schn/Fap⁺	Not Specified
+/-	E14.5	TS22: embryo	Present	Present	25 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Fap^tm1Schn/Fap⁺	Not Specified
+/+	E14.5	TS22: embryo	Present	Absent	25 µg; total RNA	Not Specified		Not Specified	Wild type data are described in the text.
M	Mol. Wt. Marker Lane
-/-	Control
+/-	Control
M	Mol. Wt. Marker Lane
-/-	Other lane(s) in blot use different probe(s)
-/-	Other lane(s) in blot use different probe(s)
+/-	Other lane(s) in blot use different probe(s)
+/-	Other lane(s) in blot use different probe(s)

Notes:
(a) This band represents the targeted allele.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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