Gene Expression Data
Assay Details
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Assay
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Reference:
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J:124331
Cho JH, et al., Requirement for Slit-1 and Robo-2 in zonal segregation of olfactory sensory neuron axons in the main olfactory bulb. J Neurosci. 2007 Aug 22;27(34):9094-104
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6693674
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Gene symbol:
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Robo2
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Gene name:
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roundabout guidance receptor 2
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Antibody:
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Anti-Robo2
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Detection system:
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Alexa Fluor 488
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Results
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Specimen
2A/C:
embryonic day 16.0
(more  )
Note: Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
Specimen
2A/C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: main olfactory bulb |
Strong |
Regionally restricted |
2C
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2A
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High level of expression in olfactory sensory neurons in dorsal and rostral regions. Large proportion of highly expressing axons originate from olfactory sensory neurons located in zone 1 of olfactory epithelium. Lower levels in subset of axons targeting ventral regions of olfactory bulb.
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Specimen
2D/F:
embryonic day 16.0
(more )
Note: Double labeled: green - Robo2; red - OCAM (Ncam2 assay MGI:6693679).
Specimen
2D/F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - OCAM (Ncam2 assay MGI:6693679).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: main olfactory bulb |
Strong |
Regionally restricted |
2F
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2D
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High level of expression in olfactory sensory neurons in dorsal and rostral regions. Large proportion of highly expressing axons originate from olfactory sensory neurons located in zone 1 of olfactory epithelium. Lower levels in subset of axons targeting ventral regions of olfactory bulb.
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Specimen
2G/I:
embryonic day 18.0
(more )
Note: Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
Specimen
2G/I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: main olfactory bulb |
Strong |
Regionally restricted |
2G
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2I
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High level of expression in olfactory sensory neurons in dorsal and rostral regions. Large proportion of highly expressing axons originate from olfactory sensory neurons located in zone 1 of olfactory epithelium. Lower levels in subset of axons targeting ventral regions of olfactory bulb.
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Specimen
2J/L:
embryonic day 18.0
(more )
Note: Double labeled: green - Robo2; red - OCAM (Ncam2 assay MGI:6693679).
Specimen
2J/L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - OCAM (Ncam2 assay MGI:6693679).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: main olfactory bulb |
Strong |
Regionally restricted |
2J
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2L
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High level of expression in olfactory sensory neurons in dorsal and rostral regions. Large proportion of highly expressing axons originate from olfactory sensory neurons located in zone 1 of olfactory epithelium. Lower levels in subset of axons targeting ventral regions of olfactory bulb.
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Specimen
2M/O:
embryonic day 18.0
(more )
Note: Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
Specimen
2M/O:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: main olfactory bulb |
Strong |
Regionally restricted |
2M
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2O
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High levels of expression in olfactory sensory neuron axons projecting to dorsal medial aspects of olfactory bulb. Large proportion of highly expressing axons originate from olfactory sensory neurons located in zone 1 of olfactory epithelium.
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Specimen
2P/R:
embryonic day 18.0
(more )
Note: Double labeled: green - Robo2; red - OCAM (Ncam2 assay MGI:6693679).
Specimen
2P/R:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - OCAM (Ncam2 assay MGI:6693679).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: main olfactory bulb |
Present |
Regionally restricted |
2P
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2R
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No expression in subset of Ncam2-expressing axons. Robo2-expressing axons restricted to nerve layer.
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Specimen
S1A/C:
embryonic day 18.0
(more )
Note: Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
Specimen
S1A/C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - Nqo1 (assay MGI:6693675).
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Specimen
S1D/F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Robo2tm1Mrt/Robo2tm1Mrt
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo2; red - OCAM (Ncam2 assay MGI:6693679).
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