Probe:	Gpc6 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Two probes were described, but the one used in this assay was not detailed. Gpc6 probe2 MGI:6515554 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS19: mandibular process mesenchyme	Strong	Regionally restricted	6A	Strong expression in mesenchyme of mandibular process with highest expression in mesenchymal cell layer just below oral epithelium.
TS19: lung mesenchyme	Strong	Regionally restricted	6A	Strong expression in mesenchyme surrounding lung bud,
TS19: dorsal aorta	Strong	Regionally restricted	6A	Strong expression in smooth muscle cells of dorsal aorta.
TS19: intestine mesenchyme	Strong	Regionally restricted	6A	Strong expression in submucosal layers of intestine.
TS19: axial mesoderm	Strong	Regionally restricted	6A	Strong expression in intervertebral disks.
TS19: otocyst	Present	Regionally restricted	6A	Expression in ventromedial wall of otic vesicle
TS19: forebrain	Present		6A
TS19: hindbrain	Weak	Regionally restricted	6A	Low levels of expression in neuroepithelium of hindbrain and telencephalic vesicle.
TS19: telencephalon	Weak	Regionally restricted	6A	Low levels of expression in neuroepithelium of hindbrain and telencephalic vesicle.
TS19: midbrain	Weak	Regionally restricted	6A	Weak expression in neuroepithelial cells lining the mesencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS19: liver	Weak	Regionally restricted		Weak expression in liver septa.
TS19: liver parenchyma	Absent
TS19: nasal mesenchyme	Strong	Regionally restricted		Strong expression in mesenchyme surrounding olfactory epithelium.
TS19: olfactory epithelium	Absent

Structure	Level	Pattern	Image	Note
TS21: intestine	Strong		6C
TS21: lung	Strong		6C
TS21: outflow tract	Strong		6C
TS21: tooth	Strong		6C

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present	Regionally restricted	6D	Expression in lung mesenchyme surrounding epithelium.
TS21: bronchus mesenchyme	Present		6D	Expression restricted to peri-bronchial mesenchyme cells.

Structure	Level	Pattern	Image	Note
TS21: dorsal aorta	Present	Regionally restricted	6E	Expression in blood vessels confined to subendothelial smooth muscle layer.
TS21: intervertebral disc	Strong		6E

Structure	Level	Pattern	Image	Note
TS21: outflow tract	Present		6F	No expression in other parts of the heart.

Structure	Level	Pattern	Image	Note
TS21: cap mesenchyme	Strong	Regionally restricted	6G	Highest expression in metanephric mesenchyme. Expression in metanephric cap mesenchyme of cortical region, in condensing mesenchyme surrounding ureteric branches.
TS21: metanephros	Present	Regionally restricted	6G	No expression in kidney epithelium.

Structure	Level	Pattern	Image	Note
TS21: stomach mesenchyme	Strong	Regionally restricted	7A	Strong expression in stomach. Expression in submucosal layers, condensing splanchnic mesenchyme, and prospective smooth muscle layer.
TS21: gonad	Present		7A

Structure	Level	Pattern	Image	Note
TS21: intestine mesenchyme	Strong	Regionally restricted	7B	Strong expression in intestine. Expression in submucosal layers, condensing splanchnic mesenchyme, and prospective smooth muscle layer.
TS21: intestine smooth muscle	Strong	Regionally restricted	7B	Strong expression in intestine. Expression in submucosal layers, condensing splanchnic mesenchyme, and prospective smooth muscle layer.

Structure	Level	Pattern	Image	Note
TS21: mesonephros	Present	Regionally restricted	7C	Expression in mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Present	Regionally restricted	7D	Expression in mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Strong	Regionally restricted	7E	Strong expression in dental mesenchyme surrounding epithelial bud.
TS21: tooth epithelium	Absent		7E	Expression limited to dental mesenchyme.
TS21: lip	Strong	Regionally restricted	7E	Very strong mesenchymal expression at top of lip furrow.
TS21: tongue	Present		7E

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Present	Regionally restricted	7F	Expression at level of caudal intervertebral disc.
TS21: vertebral cartilage condensation	Absent		7F	No expression in vertebra.
TS21: notochord	Absent		7F	No expression in remnant of notochrod.

Structure	Level	Pattern	Image	Note
TS21: nasal mesenchyme	Strong	Regionally restricted	8A	Strong expression in mesenchyme surrounding olfactory epithelium.
TS21: olfactory epithelium	Absent		8A

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Ambiguous	Regionally restricted	8B	Expression in mesenchymal tissued lining the dorsal root ganglia (perineurium), but not in the ganglia themselves.

Structure	Level	Pattern	Image	Note
TS21: cochlea epithelium	Present		8C	Expression in epithelium outlining cochlea.

Structure	Level	Pattern	Image	Note
TS21: neural retina	Present		8D
TS21: lens primordium	Present	Regionally restricted	8D	Expression in cells composing wall of lens vesicle.

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Absent
TS21: liver	Weak	Regionally restricted		Weak expression in liver septa.
TS21: liver parenchyma	Absent
TS21: pancreas mesenchyme	Present
TS21: genital tubercle	Present			Expression in genital eminence.
TS21: forelimb cartilage condensation	Strong			Highly expressed in mesenchymal condensations of both forelimb and hindlimb.
TS21: hindlimb cartilage condensation	Strong			Highly expressed in mesenchymal condensations of both forelimb and hindlimb.
TS21: neocortex	Present	Regionally restricted		Expression in roof of neopallial cortex.
TS21: choroid plexus	Weak
TS21: medulla oblongata	Weak
TS21: spinal cord mantle layer	Weak	Regionally restricted		Weak expression in ventral mantle layer of spinal cord.
TS21: glossopharyngeal ganglion	Strong			Stronger expression in ganglia of glossopharyngeal nerve.