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Gene Expression Data
Assay Details
Assay
Reference: J:296647 Wanner N, et al., DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation. J Am Soc Nephrol. 2019 Jan;30(1):63-78
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6512072
Gene symbol: Nphs1
Gene name: nephrosis 1, nephrin
Antibody: Nephrin antibody (GP-N2)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3A fl/fl - E14.5: embryonic day 14.5; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Nphs1; red - Pax2 (assay MGI:6512071)
Structure Level Pattern Image Note
TS22: visceral epithelium of maturing renal corpuscle Present 3A E14.5 fl/fl Expression is seen in podocytes.
Specimen 3A fl/fl; cre - E14.5: embryonic day 14.5; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Nphs1; red - Pax2 (assay MGI:6512071)
Structure Level Pattern Image Note
TS22: visceral epithelium of maturing renal corpuscle Present 3A E14.5 fl/fl; cre Expression is seen in podocytes. In the mutant the number of glomeruli is reduced to almost half the number seen in wild type.
Specimen 3A fl/fl - E15.5: embryonic day 15.5; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Nphs1; red - Pax2 (assay MGI:6512071)
Structure Level Pattern Image Note
TS23: visceral epithelium of maturing renal corpuscle Present 3A E15.5 fl/fl Expression is seen in podocytes.
Specimen 3A fl/fl; cre - E15.5: embryonic day 15.5; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Nphs1; red - Pax2 (assay MGI:6512071)
Structure Level Pattern Image Note
TS23: visceral epithelium of maturing renal corpuscle Present 3A E15.5 fl/fl; cre Expression is seen in podocytes. In the mutant the number of glomeruli is reduced to almost half the number seen in wild type.
Specimen 3A fl/fl - E19.5: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: green - Nphs1; red - Pax2 (assay MGI:6512071)
Structure Level Pattern Image Note
TS27: visceral epithelium of maturing renal corpuscle Present 3A E19.5 fl/fl Expression is seen in podocytes.
Specimen 3A fl/fl; cre - E19.5: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Nphs1; red - Pax2 (assay MGI:6512071)
Structure Level Pattern Image Note
TS27: visceral epithelium of maturing renal corpuscle Present 3A E19.5 fl/fl; cre Expression is seen in podocytes. In the mutant the number of glomeruli is reduced to almost half the number seen in wild type.
Specimen 3E fl/fl: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae (more )
Note: Double labeled: red - Nphs1; green - Nphs2 (podocin; assay MGI:6512077)
Structure Level Pattern Image Note
TS27: glomerular tuft Present 3E fl/fl This is a mature marker of the slit diaphragm.
Specimen 3E fl/fl; cre: postnatal day 0-1; Dnmt1tm2Jae/Dnmt1tm2Jae, Tg(Six2-EGFP/cre)1Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Nphs1; green - Nphs2 (podocin; assay MGI:6512077)
Structure Level Pattern Image Note
TS27: glomerular tuft Present 3E fl/fl; cre This is a mature marker of the slit diaphragm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory