Probe:	Mm-Spry2 (425061)
Probe preparation:	Antisense
Visualized with:	Chromogenic
Assay notes:	RNAscope assay

Structure	Level	Pattern	Image	Note
TS20: ureteric tip	Present		10A
TS20: ureteric trunk	Present		10A
TS20: metanephros	Present		10A	Some expression is seen in non-uteric bud cells.

Structure	Level	Pattern	Image	Note
TS20: ureteric tip	Present		10B	The number of transcripts per cell is the same in the mutant as in wild type.
TS20: ureteric trunk	Present		10B	The number of transcripts per cell is the same in the mutant as in wild type.
TS20: metanephros	Present		10B	Some expression is seen in non-uteric bud cells. The number of transcripts per cell is the same in the mutant as in wild type.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Non-Uniform	10E	Expression levels are higher in the tip than the stalk.
TS20: lung	Trace		10E	There is a small amount of expression in non-epithelial cells.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Non-Uniform	10F	Expression levels are higher in the tip than the stalk. The number of transcripts per cell is the same in the mutant as in wild type.
TS20: lung	Trace		10F	There is a small amount of expression in non-epithelial cells. The number of transcripts per cell is the same in the mutant as in wild type.