Antibody:	Comp antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS16: trunk mesenchyme	Present	Regionally restricted	6B , 6A	Expression is in predifferentiated mesenchyme alongside the neural tube.

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	7A	Expression is in hypertrophic chondrocytes.
TS21: rib cartilage condensation	Present	Regionally restricted	7A , 7B	Expression is in hypertrophic chondrocytes.
TS21: pre-cartilage condensation	Present	Regionally restricted	7B , 7A	Expression is in hypertrophic chondrocytes of the precartilagenous condensations.
TS21: notochord	Present		7A
TS21: skeletal muscle	Present		7A
TS21: somite	Present		7A

Structure	Level	Pattern	Image	Note
TS21: limb cartilage condensation	Present	Regionally restricted	7C	Expression is in hypertrophic chondrocytes of the precartilagenous condensations.

Structure	Level	Pattern	Image	Note
TS26: vertebral cartilage condensation	Present	Regionally restricted	8B , 8A	Expression is localized to the pericellular region and territorial extracellular matrix of hypertrophic chondrocytes and is weak in the proliferating chondrocytes in the growth zone of vertebral bodies. Expression is absent in the resting zone adjacent to the vertebral disc.

Structure	Level	Pattern	Image	Note
TS26: perichondrium	Present		8C , 8D
TS26: periosteum	Present		8C , 8D
TS26: articular cartilage	Present	Regionally restricted	8D , 8C	Expression is in the superficial layer of the articular surface. Expression is stronger in the pericellular region and territorial matrix of the hypertrophic zone and weaker in the proliferative zone and is absent in the resting zone of the humerus.
TS26: humerus	Present	Regionally restricted	8C , 8D	Expression is stronger in the pericellular region and territorial matrix of the hypertrophic zone and weaker in the proliferative zone and in the calcified matrix, and is absent in the resting zone and in the central areas of epiphyseal cartilage.

Structure	Level	Pattern	Image	Note
TS24: pre-cartilage condensation	Present	Regionally restricted		Expression is in hypertrophic chondrocytes of the precartilagenous condensations.
TS24: vertebral pre-cartilage condensation	Present	Regionally restricted		Expression is in hypertrophic chondrocytes of the precartilagenous condensations.