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Gene Expression Data
Assay Details
Assay
Reference: J:37600 Puschel AW, et al., The sensory innervation of the mouse spinal cord may be patterned by differential expression of and differential responsiveness to semaphorins. Mol Cell Neurosci. 1996 May;7(5):419-31
Assay type: RNA in situ
MGI Accession ID: MGI:6504034
Gene symbol: Sema5a
Gene name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Probe: semF probe1
Probe preparation: labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen E10.5 not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: future spinal cord Absent No expression in thoracic spinal cord at this stage.
Specimen 1G: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: neural tube floor plate Weak 1G In thoracic region, weak expression in marginal zone and floorplate.
TS19: neural tube marginal layer Weak 1G In thoracic region, weak expression in marginal zone and floorplate.
TS19: vertebral pre-cartilage condensation Present Regionally restricted 1G Authors report differential expression in prevertebrae.
Specimen 2F/H: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord thoracic region Absent 2F , 2H Expresssion disappeared at E12.5.
Specimen 4F/H: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: spinal cord dorsal horn Weak Regionally restricted 4F , 4H Low level of expression restricted to superficial dorsal horn.
TS23: trunk connective tissue Present Regionally restricted 4H , 4F Expression in connective tissues separating individual muscle masses.
Specimen E12.5 not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: telencephalon Strong Regionally restricted Strong expression in neuroepithelium of telencephalic vesicle.
Specimen 6F: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: telencephalon ventricular layer Present 6F Expression restricted to ventricular zone of telencephalon.
TS23: cerebral cortex Present 6F Expression in cortical neuroepithelium.
Specimen E15.5 not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: brain Present Regionally restricted Authors report expression in distinct cell clusters in striatum, pallidum, thalamus and tegmentum.
Specimen 7C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: telencephalon ventricular layer Present 7C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory