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Gene Expression Data
Assay Details
Assay
Reference: J:135108 Manning JA, et al., Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development. Histochem Cell Biol. 2008 Jun;129(6):751-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6503997
Gene symbol: Nedd1
Gene name: neural precursor cell expressed, developmentally down-regulated gene 1
Results
Specimen 1e: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cochlea Present 1e left , 1e right Expression is in a centrosome-like spot and immediately surrounding cytoplasm in all cells and is concentrated in the apical region of polarized cells of the cochlea.
TS22: cochlea epithelium Strong Regionally restricted 1e left , 1e right Expression is concentrated in the apical region of polarized cells.

Specimen 2e: embryonic day 14.5 (more )
Note: Double labeled: green - Nedd1; red - acetylated alpha-tubulin.
Structure Level Pattern Image Note
TS22: duodenum epithelium Present 2e Merge , 2e Nedd1 Expression is in the centrosome or basal body and in basal bodies of primary cilium.

Specimen 3a: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - Cdh2 (assay MGI:6504009).
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present Regionally restricted 3a Merge Nedd1/N-Cadherin , 3a Nedd1 , 3a Merge There is centrosomal localization at the adherins junctions of the luminal ventricular zone cells of the neural tube.

Specimen 3b: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - Cdh2 (assay MGI:6504009).
Structure Level Pattern Image Note
TS19: dorsal root ganglion Present Regionally restricted 3b Nedd1 , 3b Merge , 3b Merge Nedd1/N-Cadherin Expression is in the centrosome and in the cytoplasm of some cells of the dorsal root ganglia.

Specimen 3c: embryonic day 14.5 (more )
Note: Double labeled: green - Nedd1; red - Cdh2 (assay MGI:6504009).
Structure Level Pattern Image Note
TS22: spinal cord ventricular layer Weak 3c Merge Nedd1/N-Cadherin , 3c Nedd1 , 3c Merge

Specimen 3d: embryonic day 14.5 (more )
Note: Double labeled: green - Nedd1; red - Cdh2 (assay MGI:6504009).
Structure Level Pattern Image Note
TS22: dorsal root ganglion Weak 3d Nedd1 , 3d Merge , 3d Merge Nedd1/N-Cadherin Expression is centrosomal and cytoplasmic.

Specimen 3e: embryonic day 14.5 (more )
Note: Double labeled: green - Nedd1; red - Cdh2 (assay MGI:6504009).
Structure Level Pattern Image Note
TS22: hindbrain ventricular layer Very strong 3e Merge Nedd1/N-Cadherin , 3e Nedd1 , 3e Merge Expression is in centrosomes of proliferating cells.

Specimen 4a: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - Tubg1 (assay MGI:6504011) and Tubg2 (assay MGI:6504046).
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present Regionally restricted 4a Nedd1 , 4a Merge Expression is in centrosomes of proliferating cells.
TS19: dorsal root ganglion Present Regionally restricted 4a Nedd1 , 4a Merge Expression is centrosomal and cytoplasmic.

Specimen 4b: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - Tubg1 (assay MGI:6504011) and Tubg2 (assay MGI:6504046).
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present 4b Merge , 4b Nedd1 , 4b Nedd1/g-tubulin Expression is in centrosomes of proliferating cells.

Specimen 4c: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - Tubg1 (assay MGI:6504011) and Tubg2 (assay MGI:6504046).
Structure Level Pattern Image Note
TS19: neural tube ventricular layer Present 4c Nedd1 , 4c Nedd1/g-tubulin , 4c Merge Expression is in centrosomes of proliferating cells.
TS19: dorsal root ganglion Present 4c Nedd1/g-tubulin , 4c Merge , 4c Nedd1 Expression is centrosomal and cytoplasmic.

Specimen 5a: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - Tubg1 (assay MGI:6504011) and Tubg2 (assay MGI:6504046).
Structure Level Pattern Image Note
TS19: lens vesicle epithelium Present Regionally restricted 5a Nedd1 , 5a Merge , 5a Nedd1/g-tubulin Expression is in centrosomes in the apical region of polarized epithelial cells.

Specimen 5b: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - Tubg1 (assay MGI:6504011) and Tubg2 (assay MGI:6504046).
Structure Level Pattern Image Note
TS19: neural retina Present Regionally restricted 5b Merge , 5b Nedd1/g-tubulin , 5b Nedd1 Expression is at the subventricular region of polarized invaginated neural ectoderm cells in the retina.

Specimen 5c: embryonic day 14.5 (more )
Note: Double labeled: green - Nedd1; red - Tubg1 (assay MGI:6504011) and Tubg2 (assay MGI:6504046).
Structure Level Pattern Image Note
TS22: lens epithelium Present Regionally restricted 5c Merge , 5c Nedd1 , 5c Nedd1/g-tubulin Expression is in polarized lens epithelial cells at the boundary of the lens fiber cells colocalizing with gamma-tubulin.

Specimen 5d: embryonic day 14.5 (more )
Note: Double labeled: green - Nedd1; red - Tubg1 (assay MGI:6504011) and Tubg2 (assay MGI:6504046).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 5d Nedd1/g-tubulin , 5d Merge , 5d Nedd1 Expression is in a polarized pattern in the subventricular region of cells of the outer neuroblastic layer.

Specimen 6a: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - acetylated alpha-tubulin.
Structure Level Pattern Image Note
TS19: lens vesicle epithelium Present Regionally restricted 6a Merge , 6a Nedd1 , 6a Nedd1/Acet a-tubulin Expression is at the base of the cilia.

Specimen 6b: embryonic day 11.5 (more )
Note: Double labeled: green - Nedd1; red - acetylated alpha-tubulin.
Structure Level Pattern Image Note
TS19: neural retina Present Regionally restricted 6b Nedd1 , 6b Merge , 6b Nedd1/Acet a-tubulin Expression is at the base of the cilia.

Specimen 7a: embryonic day 15.5 (more )
Note: Double labeled: green - Nedd1; red - phallodin.
Structure Level Pattern Image Note
TS23: lung epithelium Present 7a Nedd1 , 7a Nedd1/Phallodin , 7a Merge Expression is in the centrosomes at the apical surface.

Specimen 7b: embryonic day 15.5 (more )
Note: Double labeled: green - Nedd1; red - phallodin.
Structure Level Pattern Image Note
TS23: duodenum epithelium Present 7b Nedd1 , 7b Nedd1/Phallodin , 7b Merge Expression is in the centrosomes at the apical surface.

Specimen 7c: embryonic day 15.5 (more )
Note: Double labeled: green - Nedd1; red - phallodin.
Structure Level Pattern Image Note
TS23: cochlea epithelium Present 7c Nedd1/Phallodin , 7c Nedd1 , 7c Merge Expression is in the centrosomes at the apical surface.

Specimen not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: brain ventricular layer Present Regionally restricted There is centrosomal localization at the adherins junctions of the luminal ventricular zone cells.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory