Gene Expression Data
Assay Details
Assay
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Reference:
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J:70696
Gowan K, et al., Crossinhibitory activities of ngn1 and math1 allow specification of distinct dorsal interneurons. Neuron. 2001 Aug;31(2):219-32
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6502156
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Antibody:
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anti-MASH1
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Detection system:
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Secondary antibody
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Results
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Specimen
1C:
embryonic day 10.5
(more )
Note: Dorsal boundaries. Triple labeled: lower red - Ascl1; green - Neurog1 (assay MGI:6502158); upper red - Atoh1 (assay MGI:6502147).
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dorsal boundaries. Triple labeled: lower red - Ascl1; green - Neurog1 (assay MGI:6502158); upper red - Atoh1 (assay MGI:6502147).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube lateral wall |
Present |
Regionally restricted |
1C
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Expression of Atoh1, Neurog1, and Ascl1 define three distinct, nonoverlapping populations of neural progenitors.
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Specimen
1D:
embryonic day 11.5
(more )
Note: Dorsal boundaries. Triple labeled: lower red - Ascl1; green - Neurog1 (assay MGI:6502158); upper red - Atoh1 (assay MGI:6502147).
Specimen
1D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Dorsal boundaries. Triple labeled: lower red - Ascl1; green - Neurog1 (assay MGI:6502158); upper red - Atoh1 (assay MGI:6502147).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
1D
|
Expression of Atoh1, Neurog1, and Ascl1 define three distinct, nonoverlapping populations of neural progenitors.
|
Specimen
1E:
embryonic day 10.5
(more )
Note: Ventral boundaries. Double labeled: red - Ascl1; green - Neurog1 (assay MGI:6502158).
Specimen
1E:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 10.5
|
Sex:
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Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Ventral boundaries. Double labeled: red - Ascl1; green - Neurog1 (assay MGI:6502158).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube lateral wall |
Present |
Regionally restricted |
1E
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No coexpression of Neurog1 and Ascl1 in neural progenitors.
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Specimen
1F:
embryonic day 11.5
(more )
Note: Ventral boundaries. Double labeled: red - Ascl1; green - Neurog1 (assay MGI:6502158).
Specimen
1F:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Ventral boundaries. Double labeled: red - Ascl1; green - Neurog1 (assay MGI:6502158).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
1F
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No coexpression of Neurog1 and Ascl1 in neural progenitors.
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