Gene Expression Data
Assay Details
Assay
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Reference:
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J:70696
Gowan K, et al., Crossinhibitory activities of ngn1 and math1 allow specification of distinct dorsal interneurons. Neuron. 2001 Aug;31(2):219-32
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6502147
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Gene symbol:
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Atoh1
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Gene name:
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atonal bHLH transcription factor 1
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Results
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Specimen
1C:
embryonic day 10.5
(more )
Note: Dorsal boundaries. Triple labeled: upper red - Atoh1; green - Neurog1 (assay MGI:6502158); lower red - Ascl1 (assay MGI:6502156).
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dorsal boundaries. Triple labeled: upper red - Atoh1; green - Neurog1 (assay MGI:6502158); lower red - Ascl1 (assay MGI:6502156).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: neural tube lateral wall |
Present |
Regionally restricted |
1C
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Expression of Atoh1, Neurog1, and Ascl1 define three distinct, nonoverlapping populations of neural progenitors. The domain was more extensive than at E11.5.
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Specimen
1D:
embryonic day 11.5
(more )
Note: Dorsal boundaries. Triple labeled: upper red - Atoh1; green - Neurog1 (assay MGI:6502158); lower red - Ascl1 (assay MGI:6502156).
Specimen
1D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Dorsal boundaries. Triple labeled: upper red - Atoh1; green - Neurog1 (assay MGI:6502158); lower red - Ascl1 (assay MGI:6502156).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
1D
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Expression of Atoh1, Neurog1, and Ascl1 define three distinct, nonoverlapping populations of neural progenitors.
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Specimen
6G:
embryonic day 11.5
(more )
Note: Double labeled: red - Atoh1; green - Ascl1 (assay MGI:6502157).
Specimen
6G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Atoh1; green - Ascl1 (assay MGI:6502157).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
6G
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Expression domains of Atoh1 and Ascl1 were distinct with a clear space separating them.
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Specimen
6H:
(close )
Genetic Background:
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involves: 129S1/Sv * 129S7/SvEvBrd * 129X1/SvJ * ICR
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Mutant Allele(s):
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Neurog1tm1And/Neurog1tm1And Neurog2tm1Fgu/Neurog2tm1Fgu
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Atoh1; green - Ascl1 (assay MGI:6502157).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube lateral wall |
Present |
Regionally restricted |
6H
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Expression domain was clearly expanded ventrally to the dorsal border of the Ascl1 domain. The number of positive cells was increased.
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