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Gene Expression Data
Assay Details
Assay
Reference: J:79434 Loomes KM, et al., Characterization of Notch receptor expression in the developing mammalian heart and liver. Am J Med Genet. 2002 Oct 1;112(2):181-9
Assay type: RT-PCR
MGI Accession ID: MGI:6501987
Gene symbol: Notch3
Gene name: notch 3
Probe: Notch3-pG, Notch3-pH
Probe preparation: labelled with FAM
Assay notes: This assay is a quantitative RT-PCR. All expression levels were relative to levels detected at E11.5, and normalized with respect to Gapdh expression. (FAM)CTCATCGCCAGCCATGCCGA(TAMRA) was used as the probe.
Results Image: 1C
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
E11 E11.5 TS19: heart Weak 1 µg CD-1 Not Specified
E12 E12.5 TS20: heart Weak 1 µg CD-1 Not Specified
E13 E13.5 TS21: heart Weak 1 µg CD-1 Not Specified
E15 E15.5 TS23: heart Weak 1 µg CD-1 Not Specified
E18 E18.5 TS26: heart Weak 1 µg CD-1 Not Specified
NB P newborn TS27: heart Weak 1 µg CD-1 Not Specified
2WK P w 2 TS28: heart Present 1 µg CD-1 Not Specified
12WK P w 12 TS28: heart Present (b) 1 µg CD-1 Not Specified
Notes:
(a) Expression remained at low levels throughout gestation and rised sharply by 12 weeks of age.
(b) About 9 times the initial level.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory