Trib3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:215219 Ord T, et al., Trib3 is developmentally and nutritionally regulated in the brain but is dispensable for spatial memory, fear conditioning and sensing of amino acid-imbalanced diet. PLoS One. 2014;9(4):e94691
Assay type:	RT-PCR
MGI Accession ID:	MGI:6478216
Gene symbol:	Trib3
Gene name:	tribbles pseudokinase 3

Probe:	Trib3-pE, Trib3-pF
Assay notes:	Quantitative RT-PCR. The mean expression level at the indicated age is presented relative to the level of Trib3 expression at E14. Ribosomal protein L7a (Rpl7a) was used as the endogenous reference gene.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	102 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E14	E14.0 (a)	TS22: brain	Present	0.5 µg; total RNA	Not Specified		Not Specified
E15	E15.0 (a)	TS23: brain	Present	0.5 µg; total RNA	Not Specified		Not Specified
E17	E17.0 (a)	TS25: brain	Present	0.5 µg; total RNA	Not Specified		Not Specified
E18	E18.0 (a)	TS26: brain	Present	0.5 µg; total RNA	Not Specified		Not Specified
P0	P0	TS27: brain	Present	0.5 µg; total RNA	Not Specified		Not Specified
P2	P2	TS27: brain	Present	0.5 µg; total RNA	Not Specified		Not Specified
P4	P4	TS28: brain	Present	0.5 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression increased steadily from E14 to E18 by approximately 4-fold. After birth (P0), the level of Trib3 mRNA is decreased by approximately 50% compared to E18, however, this decrease appears to be transient, as a resumption of elevated Trib3 expression is evident at over following days, with a peak at P2 reaching nearly 6-fold higher than E14.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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