About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:289834 Ishiuchi T, et al., Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Cell Rep. 2019 May 7;27(6):1742-1754.e6
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6477560
Gene symbol: Zfp281
Gene name: zinc finger protein 281
Antibody: anti-ZNF281 (sc-166933)
Detection system: Secondary antibody
Assay notes: The authors did not specify which antibody was used. It is possible anti-Zfp281 (ab101318) (MGI:6476905) was used.
Results
Specimen 3E E6.5: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 3E E6.5 Zfp281 , 3E E6.5 2 , 3E E6.5 merge , 3E E6.5 1
TS9: ectoplacental cone Weak 3E E6.5 merge , 3E E6.5 2 , 3E E6.5 1 , 3E E6.5 Zfp281
Specimen 3E E7.5: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: extraembryonic ectoderm Present 3E E7.5 Merge , 3E E7.5 2 , 3E E7.5 1 , 3E E7.5 Zfp281
TS11: ectoplacental cone Present 3E E7.5 1 , 3E E7.5 Merge , 3E E7.5 2 , 3E E7.5 Zfp281
Specimen S3E: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: primitive endoderm Absent S3E Zfp281 left , S3E Zfp281 Merge right , S3E Zfp281 Merge left , S3E Zfp281 right
Specimen S3F Control: embryonic day 6.5 (more )
Note: Also stained with Phalloidin (green).
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present S3F control
TS9: ectoplacental cone Weak S3F control
Specimen S3F Zfp281 KO: embryonic day 6.5; Zfp281em1Hsas/Zfp281em1Hsas (more )
Note: Also stained with Phalloidin (green). The authors stated identical results were obtained with both mutants; both have been annotated.
Structure Level Pattern Image Note
TS9: ectoplacental cone Absent S3F KO
TS9: extraembryonic ectoderm Absent S3F KO
Specimen S3F KO: embryonic day 6.5; Zfp281em2Hsas/Zfp281em2Hsas (more )
Note: The authors stated identical results were obtained with both mutants; both have been annotated.
Structure Level Pattern Image Note
TS9: ectoplacental cone Absent
TS9: extraembryonic ectoderm Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory