Antibody:	anti-Lamin A/C (mAB346)
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Expression was summarized in Table 1.

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Strong		2A	Expressed in the nuclei.
TS28: decidua	Strong		2A	Expressed in the nuclei.
TS15: yolk sac	Trace		2A	The majority of yolk sac nuclei were negative.

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent		2B

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present		2H , 2G	Expressed in a minority of myoblasts.

Structure	Level	Pattern	Image	Note
TS21: eye muscle	Strong		3B

Structure	Level	Pattern	Image	Note
TS23: epidermis	Weak	Regionally restricted	3F	The most differentiated outer layer was relatively strongly stained, the basal layer less strongly while cells of the epidermal primordia were not stained.
TS23: dermis	Strong		3F	Expressed in the nuclei.
TS23: muscle tissue	Strong		3F	Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS27: connective tissue	Present		4B
TS27: brain	Absent		4B

Structure	Level	Pattern	Image	Note
TS28: heart	Strong		4E	Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Strong		4F	Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Strong		4G	Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS28: liver	Strong		4H	Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS28: intestine epithelium	Weak		4I	Expressed in the nuclei. Not all epithelial cells are positively stained.

Structure	Level	Pattern	Image	Note
TS28: brain	Strong		4J	Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Absent
TS15: umbilical blood vessel	Absent

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Trace			The majority of yolk sac nuclei were negative.
TS17: trophoblast giant cell	Strong
TS28: decidua	Strong
TS17: dorsal aorta	Absent
TS17: embryo	Absent
TS17: umbilical blood vessel	Absent

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Present	Single cells		Diffuse staining in the cytoplasm of a few mesenchymal cells.
TS19: limb mesenchyme	Present	Single cells		Diffuse staining in the cytoplasm of a few presumptive myoblasts.
TS19: dorsal aorta	Absent
TS19: umbilical blood vessel	Absent

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Trace	Single cells		occasional cells
TS20: eye mesenchyme	Present			Expressed in a minority of myoblasts.
TS20: trunk musculature	Present			Expressed in a minority of myoblasts.
TS20: heart	Absent
TS20: umbilical blood vessel	Weak
TS20: dorsal aorta	Weak			Very weak.

Structure	Level	Pattern	Image	Note
TS21: limb muscle	Strong
TS21: trunk musculature	Strong
TS21: heart	Absent
TS21: embryo mesenchyme	Trace	Single cells		occasional cells
TS21: dorsal aorta	Weak
TS21: umbilical blood vessel	Weak

Structure	Level	Pattern	Image	Note
TS22: embryo mesenchyme	Present			Expressed in a minority of cells.
TS22: eye muscle	Strong
TS22: limb muscle	Strong
TS22: trunk musculature	Strong
TS22: heart	Absent
TS22: dorsal aorta	Weak
TS22: umbilical blood vessel	Weak
TS22: epidermis	Present			Diffuse staining in the cytoplasm of a minority of cells.

Structure	Level	Pattern	Image	Note
TS23: embryo mesenchyme	Strong
TS23: eye muscle	Strong
TS23: limb muscle	Strong
TS23: trunk musculature	Strong
TS23: heart	Absent
TS23: aorta	Weak
TS23: umbilical blood vessel	Weak

Structure	Level	Pattern	Image	Note
TS24: embryo mesenchyme	Strong
TS24: epidermis	Strong
TS24: eye muscle	Strong
TS24: limb muscle	Strong
TS24: trunk musculature	Strong
TS24: heart	Absent
TS24: aorta	Moderate
TS24: umbilical blood vessel	Moderate

Structure	Level	Pattern	Image	Note
TS25: embryo mesenchyme	Strong
TS25: epidermis	Strong
TS25: eye muscle	Strong
TS25: limb muscle	Strong
TS25: trunk musculature	Strong
TS25: aorta	Moderate
TS25: tongue epithelium	Moderate
TS25: umbilical blood vessel	Moderate
TS25: heart	Absent

Structure	Level	Pattern	Image	Note
TS27: aorta	Moderate
TS27: lung epithelium	Present			Expressed in a minority of cells.
TS27: intestine epithelium	Absent
TS27: nasopharynx epithelium	Absent

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Strong
TS28: epidermis	Strong
TS28: lung epithelium	Strong
TS28: skeletal muscle	Strong
TS28: brain	Present			Expressed in a minority of cells.
TS28: heart	Present			Expressed in a minority of cells.
TS28: liver	Present			Expressed in a minority of cells.
TS28: nasopharynx epithelium	Present			Expressed in a minority of cells.
TS28: renal tubule epithelium	Present			Expressed in a minority of cells.
TS28: aorta	Moderate
TS28: intestine epithelium	Absent

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Strong
TS28: epidermis	Strong
TS28: liver	Strong
TS28: lung epithelium	Strong
TS28: skeletal muscle	Strong
TS28: aorta	Moderate
TS28: heart	Moderate
TS28: renal tubule epithelium	Moderate
TS28: nasopharynx epithelium	Weak
TS28: brain	Present			Expressed in a minority of cells.
TS28: intestine epithelium	Absent

Structure	Level	Pattern	Image	Note
TS28: brain	Strong
TS28: connective tissue	Strong
TS28: epidermis	Strong
TS28: heart	Strong
TS28: lung epithelium	Strong
TS28: renal tubule epithelium	Strong
TS28: skeletal muscle	Strong
TS28: aorta	Moderate
TS28: liver	Moderate
TS28: nasopharynx epithelium	Moderate
TS28: spleen	Present			Expressed in a minority of cells.
TS28: thymus	Present			Expressed in a minority of cells.
TS28: intestine epithelium	Absent

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Strong
TS28: epidermis	Strong
TS28: aorta	Moderate
TS28: tongue epithelium	Moderate
TS28: bone marrow	Present			Expressed in a minority of cells.
TS28: spleen	Present			Expressed in a minority of cells.
TS28: thymus	Present			Expressed in a minority of cells.