Mki67 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:257896 Huang W, et al., Loss of microRNA-128 promotes cardiomyocyte proliferation and heart regeneration. Nat Commun. 2018 Feb 16;9(1):700
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6470568
Gene symbol:	Mki67
Gene name:	antigen identified by monoclonal antibody Ki 67

Antibody:	Anti-Ki67 (ab15580)
Detection system:	Secondary antibody coupled to fluorescein

Results

1a P1 ©

Specimen 1a P1: postnatal day 1 (more

)
Note: Double labeled: green - Mki67; red - Tnnt2 (assay MGI:6470553).

Structure	Level	Pattern	Image	Note
TS27: myocardium layer	Present		1a P1

1a P7 ©

Specimen 1a P7: postnatal day 7 (more

)
Note: Double labeled: green - Mki67; red - Tnnt2 (assay MGI:6470553).

Structure	Level	Pattern	Image	Note
TS28: myocardium layer	Present		1a P7

1a P28 ©

Specimen 1a P28: postnatal day 28 (more

)
Note: Double labeled: green - Mki67; red - Tnnt2 (assay MGI:6470553).

Structure	Level	Pattern	Image	Note
TS28: myocardium layer	Present	Single cells	1a P28	Expression decreased with age.

3g G-1 ©

Specimen 3g Ctrl: postnatal day 7; Mir128-2^tm1.1Ygw/Mir128-2^tm1.1Ygw (more

)
Note: Double labeled: green - Mki67; red - Tnnt2 (assay MGI:6470553).

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present	Single cells	3g G-1

3g G-2 ©

3g G-3 ©

3g G-5 ©

Specimen 3g miR-128-/-: postnatal day 7; Mir128-2^tm1.1Ygw/Mir128-2^tm1.1Ygw, Tg(Nkx2-5-cre)9Eno/0 (more

)
Note: Conditional mutant. Double labeled: green - Mki67; red - Tnnt2 (assay MGI:6470553).

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present		3g G-3 , 3g G-5 , 3g G-2	The number of positve cells has increased in this mutant compared to controls.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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