Wnt16 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:177133 Mehta V, et al., Atlas of Wnt and R-spondin gene expression in the developing male mouse lower urogenital tract. Dev Dyn. 2011 Nov;240(11):2548-60
Assay type:	RNA in situ
MGI Accession ID:	MGI:6470465
Gene symbol:	Wnt16
Gene name:	wingless-type MMTV integration site family, member 16

Probe:	CVprobeWnt16(0038)
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

8G ©

Specimen 8G: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the urothelium and Acta2 (green) to visualize the muscularis mucosa and muscularis propria.

Structure	Level	Image	Note
TS25: epithelium of pelvic urethra of female	Present	8G	Expression is present in the urothelium.
TS25: lamina propria of pelvic urethra of female	Absent	8G
TS25: submucosa of pelvic urethra of female	Absent	8G

8H ©

Specimen 8H: embryonic day 17.5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the urothelium and Acta2 (green) to visualize the muscularis mucosa and muscularis propria.

Structure	Level	Pattern	Image	Note
TS25: lamina propria of pelvic urethra of male	Present	Regionally restricted	8H	Expression is restricted to the caudal aspect of the pelvic urethra.
TS25: submucosa of pelvic urethra of male	Present	Regionally restricted	8H	Expression is restricted to the caudal aspect of the pelvic urethra.
TS25: epithelium of pelvic urethra of male	Absent		8H

8I ©

Specimen 8I: postnatal day 5 (more

)
Note: Labeling was visualized using BM purple. The specimen was also stained for Cdh1 (red) to visualize the urothelium and Acta2 (green) to visualize the muscularis mucosa and muscularis propria.

Structure	Level	Pattern	Image	Note
TS28: lamina propria of pelvic urethra of male	Present	Regionally restricted	8I	Expression is restricted to the caudal aspect of the pelvic urethra.
TS28: submucosa of pelvic urethra of male	Present	Regionally restricted	8I	Expression is restricted to the caudal aspect of the pelvic urethra.
TS28: epithelium of pelvic urethra of male	Absent		8I

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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