About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:187370 Keil KP, et al., Visualization and quantification of mouse prostate development by in situ hybridization. Differentiation. 2012 Oct;84(3):232-9
Assay type: RNA in situ
MGI Accession ID: MGI:6470421
Gene symbol: Edar
Gene name: ectodysplasin-A receptor
Probe: CVprobeEdar(0166)
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 17.5 (more )
Note: The specimen was also stained for Cdh1 (red) to visualize the epithelium and Acta2 (green) to visualize the smooth muscle.
Structure Level Pattern Image Note
TS25: prostate bud Present Regionally restricted 1A Expression is seen in the bud tips.
TS25: epithelium of pelvic urethra of male Absent 1A
TS25: mesenchymal layer of pelvic urethra of male Absent 1A No expression is detected in the stroma.

Specimen 1B: embryonic day 17.5 (more )
Note: The specimen was also stained for Cdh1 (red) to visualize the epithelium and Acta2 (green) to visualize the smooth muscle.
Structure Level Pattern Image Note
TS25: pelvic urethra of female Absent 1B Expression is not detected in any female urogenital sinus tissue compartment.
TS25: vagina Absent 1B Expression is not detected in any female urogenital sinus tissue compartment.

Specimen 2B: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: epithelium of pelvic urethra of male Present Widespread 2B Diffuse staining is seen throughout the urogenital sinus epithelium.

Specimen 2E: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: epithelium of pelvic urethra of male Present Regionally restricted 2E Expression is seen in a diffuse pattern throughout the urogenital sinus epithelium.

Specimen 2H: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: anterior prostate bud Present 2H
TS25: dorsal prostate bud Present 2H
TS25: lateral prostate bud Present 2H
TS25: ventral prostate bud Present 2H

Specimen 2K: embryonic day 18.5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS26: anterior prostate bud Present 2K
TS26: dorsal prostate bud Present 2K
TS26: lateral prostate bud Present 2K
TS26: ventral prostate bud Present 2K
TS26: mesenchymal layer of pelvic urethra of male Present Regionally restricted 2K Diffuse staining is seen in the urethral mesenchyme.
TS26: male urethral gland bud Absent 2K No expression is seen in the epithelium.

Specimen 2K - inset: embryonic day 18.5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS26: prostate bud Present Regionally restricted 2K inset Expression is not detected in the main prostatic bud; instead it is localized to bud tips distal to primary and secondary branch points.

Specimen 2N: postnatal day 5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS28: prostate gland Present 2N
TS28: male urethral gland Absent 2N

Specimen 2N - inset: postnatal day 5 (more )
Note: This specimen was also stained with an antibody against Cdh1 (brown) to visualize the epithelium.
Structure Level Pattern Image Note
TS28: prostate epithelium Present Regionally restricted 2N inset Expression is not detected in the main prostatic bud; instead it is localized to bud tips distal to primary and secondary branch points.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
04/23/2024
MGI 6.23
The Jackson Laboratory