About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:36870 Wilkinson GA, et al., Neurotrophin-3 is a survival factor in vivo for early mouse trigeminal neurons. J Neurosci. 1996 Dec 1;16(23):7661-9
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6468026
Gene symbol: Ntf3
Gene name: neurotrophin 3
Reporter: lacZ
Direct Detection
Results
Specimen 3A: embryonic day 9.5; Ntf3tm1Lfr/Ntf3+ (more )
Structure Level Pattern Image Note
TS15: 1st branchial arch Present 3A
TS15: future midbrain Present Regionally restricted 3A Expression is seen in the anterior and posterior neuropores. There is strong staining at the midline of the mesencephalon roof.

Specimen 3B: embryonic day 10.5; Ntf3tm1Lfr/Ntf3+ (more )
Note: Double labeled: blue - Ntf3 reporter; red - Tubb3 (assay MGI:6468025)
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component Present Widespread 3B Expression is throughout the developing mandibular and maxillary processes.
TS17: 1st branchial arch maxillary component Present Widespread 3B Expression is throughout the developing mandibular and maxillary processes.

Specimen not shown: embryonic day 10.5; Ntf3tm1Lfr/Ntf3+ (more )
Structure Level Pattern Image Note
TS17: olfactory placode Present Staining of intermediate intensity is seen in the olfactory pit.
TS17: optic cup Present Staining of intermediate intensity is seen in the eye cup.

Specimen 3C: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Structure Level Pattern Image Note
TS19: maxillary process Present Non-Uniform 3C Expression is strongest at the distal half of the maxillary process.

Specimen not shown: embryonic day 11.5; Ntf3tm1Lfr/Ntf3+ (more )
Structure Level Pattern Image Note
TS19: mandibular process Present Non-Uniform Expression is strongest at the distal half of the mandibular process.

Specimen 3D: embryonic day 13.5; Ntf3tm1Lfr/Ntf3+ (more )
Structure Level Pattern Image Note
TS21: mystacial pad Present 3D Staining is strongest in the mystacial pad; it is less distinct in the surrounding regions.

Specimen not shown: embryonic day 13.5; Ntf3tm1Lfr/Ntf3+ (more )
Structure Level Pattern Image Note
TS21: naris Strong Intense staining in the nostrils.
TS21: ear Strong
TS21: perioptic mesenchyme Strong Intense staining in the mesenchyme surrounding the eyeball.

Specimen 4A: embryonic day 11.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS19: maxillary process Present Widespread 4A Staining is seen in nearly every cell in the distal end of the maxillary process.
TS19: head surface ectoderm Strong Regionally restricted 4A Expression is seen in the presumptive epidermis of the maxillary process.
TS19: head mesenchyme derived from neural crest Strong Regionally restricted 4A Expression is seen in the superficial (derived from neural crest) mesenchyme of the maxillary process.
TS19: mesenchyme derived from head mesoderm Strong Regionally restricted 4A Expression is seen in the deep (derived from mesoderm) mesenchyme of the maxillary process.

Specimen 4B: embryonic day 11.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Note: Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:6468025)
Structure Level Pattern Image Note
TS19: maxillary process Present Widespread 4B Nearly every cell in the distal end of the maxillary process is stained.

Specimen not shown: embryonic day 11.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS19: cornea epithelium Present Staining is seen in the corneal ectoderm.
TS19: nasal pit Present Staining is seen in the nostrils.
TS19: oral epithelium Present
TS19: mandibular process Present Widespread Staining is seen in nearly every cell in the distal end of the mandibular process.

Specimen 4C: embryonic day 12.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS20: maxillary process mesenchyme Strong 4C
TS20: maxillary process epithelium Present 4C Staining in the skin is weakening relative to earlier levels.

Specimen 4D: embryonic day 12.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Note: Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:6468025)
Structure Level Pattern Image Note
TS20: head mesenchyme Present 4D Staining is seen in the mesenchyme surrounding the anterior edge of the trigeminal.
TS20: trigeminal ganglion Present Single cells 4D Staining is seen in a few non-neuronal cells within the ganglion.

Specimen 4E: embryonic day 13.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS21: mystacial pad Present Regionally restricted 4E Expression is confined to the mesenchyme underlying the skin (and the surrounding whisker follicles).

Specimen 4F: embryonic day 13.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS21: mystacial pad Present Regionally restricted 4F Expression is confined to the mesenchyme surrounding the whisker follicles (and underlying the skin of the mystacial pad).

Specimen not shown: embryonic day 13.5; Ntf3tm1Lfr/Ntf3tm1Lfr (more )
Structure Level Pattern Image Note
TS21: eye Present Staining is seen in the eyeball.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
04/23/2024
MGI 6.23
The Jackson Laboratory