Gene Expression Data
Assay Details
Assay
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Reference:
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J:36870
Wilkinson GA, et al., Neurotrophin-3 is a survival factor in vivo for early mouse trigeminal neurons. J Neurosci. 1996 Dec 1;16(23):7661-9
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6468026
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Gene symbol:
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Ntf3
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Gene name:
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neurotrophin 3
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
3A:
embryonic day 9.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
3A:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: 1st branchial arch |
Present |
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3A
|
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TS15: future midbrain |
Present |
Regionally restricted |
3A
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Expression is seen in the anterior and posterior neuropores. There is strong staining at the midline of the mesencephalon roof.
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Specimen
3B:
embryonic day 10.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; red - Tubb3 (assay MGI:6468025)
Specimen
3B:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Note:
|
Double labeled: blue - Ntf3 reporter; red - Tubb3 (assay MGI:6468025)
|
Specimen
not shown:
embryonic day 10.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: olfactory placode |
Present |
|
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Staining of intermediate intensity is seen in the olfactory pit.
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TS17: optic cup |
Present |
|
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Staining of intermediate intensity is seen in the eye cup.
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Specimen
3C:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
3C:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: maxillary process |
Present |
Non-Uniform |
3C
|
Expression is strongest at the distal half of the maxillary process.
|
Specimen
not shown:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: mandibular process |
Present |
Non-Uniform |
|
Expression is strongest at the distal half of the mandibular process.
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Specimen
3D:
embryonic day 13.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
3D:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: mystacial pad |
Present |
|
3D
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Staining is strongest in the mystacial pad; it is less distinct in the surrounding regions.
|
Specimen
not shown:
embryonic day 13.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Specimen
4A:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
2% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
4B:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:6468025)
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: maxillary process |
Present |
Widespread |
4B
|
Nearly every cell in the distal end of the maxillary process is stained.
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4C:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
|
section from whole mount
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Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4D:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:6468025)
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head mesenchyme |
Present |
|
4D
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Staining is seen in the mesenchyme surrounding the anterior edge of the trigeminal.
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TS20: trigeminal ganglion |
Present |
Single cells |
4D
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Staining is seen in a few non-neuronal cells within the ganglion.
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Specimen
4E:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: mystacial pad |
Present |
Regionally restricted |
4E
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Expression is confined to the mesenchyme underlying the skin (and the surrounding whisker follicles).
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Specimen
4F:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: mystacial pad |
Present |
Regionally restricted |
4F
|
Expression is confined to the mesenchyme surrounding the whisker follicles (and underlying the skin of the mystacial pad).
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: eye |
Present |
|
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Staining is seen in the eyeball.
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