Reporter:	lacZ
	Direct Detection
Assay notes:	Fixation with 1% formaldehyde, 0.2% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS15: trigeminal preganglion	Trace		2A	Faint expression in area of trigeminal ganglion.

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Strong		2B	Strong expression in neural crest-derived cranial nerve ganglia V, VII, IX and X.
TS17: glossopharyngeal ganglion	Strong		2B	Strong expression in neural crest-derived cranial nerve ganglia V, VII, IX and X.
TS17: trigeminal ganglion	Strong		2B	Strong expression in neural crest-derived cranial nerve ganglia V, VII, IX and X.
TS17: vagus ganglion	Strong		2B	Strong expression in neural crest-derived cranial nerve ganglia V, VII, IX and X.
TS17: genital swelling	Present		2B	Expression in external genitalia.

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Strong		2C	Strong expression in neural crest-derived cranial nerve ganglia.
TS17: glossopharyngeal ganglion	Strong		2C	Strong expression in neural crest-derived cranial nerve ganglia.
TS17: trigeminal ganglion	Strong		2C	Strong expression in neural crest-derived cranial nerve ganglia.
TS17: vagus ganglion	Strong		2C	Strong expression in neural crest-derived cranial nerve ganglia.
TS17: genital swelling	Present		2C	Expression in external genitalia.

Structure	Level	Pattern	Image	Note
TS19: facial ganglion	Very strong		2D	Expression remains prominent in cranial nerve ganglia. Strongest expression at E11.5
TS19: glossopharyngeal ganglion	Very strong		2D	Expression remains prominent in cranial nerve ganglia. Strongest expression at E11.5
TS19: trigeminal ganglion	Very strong		2D	Expression remains prominent in cranial nerve ganglia. Strongest expression at E11.5
TS19: vagus ganglion	Very strong		2D	Expression remains prominent in cranial nerve ganglia. Strongest expression at E11.5
TS19: dorsal root ganglion	Present		2D
TS19: genital swelling of genital tubercle	Strong		2D	Strong expression in external genitalia.

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Weak		2E	Expression diminishes in cranial nerve ganglia.
TS21: vagus ganglion	Ambiguous		2E
TS21: choroid plexus	Present		2E
TS21: adrenal gland	Present		2E
TS21: male external genitalia	Strong		2E

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Weak		2F	Expression diminishes in cranial nerve ganglia.
TS22: vagus ganglion	Ambiguous		2F
TS22: choroid plexus	Present		2F
TS22: adrenal gland	Present		2F
TS22: male external genitalia	Strong		2F

Structure	Level	Pattern	Image	Note
TS19: facial ganglion	Strong		3A	Strong expression in cranial nerve ganglia V, VII, IX and X.
TS19: glossopharyngeal ganglion	Strong		3A	Strong expression in cranial nerve ganglia V, VII, IX and X.
TS19: trigeminal ganglion	Strong		3A	Strong expression in cranial nerve ganglia V, VII, IX and X.
TS19: vagus ganglion	Strong		3A	Strong expression in cranial nerve ganglia V, VII, IX and X.

Structure	Level	Pattern	Image	Note
TS19: facial ganglion	Present		3B
TS19: acoustic ganglion	Absent		3B

Structure	Level	Pattern	Image	Note
TS19: glossopharyngeal ganglion	Present	Regionally restricted	3C	Continuous expression in cranial ganglia IX and X along cranial-caudal axis.
TS19: vagus ganglion	Present	Regionally restricted	3C	Continuous expression in cranial ganglia IX and X along cranial-caudal axis.

Structure	Level	Pattern	Image	Note
TS19: glossopharyngeal ganglion	Present	Regionally restricted	3D	Continuous expression in cranial ganglia IX and X along cranial-caudal axis.
TS19: vagus ganglion	Present	Regionally restricted	3D	Continuous expression in cranial ganglia IX and X along cranial-caudal axis.

Structure	Level	Pattern	Image	Note
TS19: glossopharyngeal ganglion	Present	Regionally restricted	3E	Continuous expression in cranial ganglia IX and X along cranial-caudal axis.
TS19: vagus ganglion	Present	Regionally restricted	3E	Continuous expression in cranial ganglia IX and X along cranial-caudal axis.

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Strong		3F	Strong expression in cranial nerve ganglia V, VII, IX and X.
TS22: glossopharyngeal ganglion	Strong		3F	Strong expression in cranial nerve ganglia V, VII, IX and X.
TS22: trigeminal ganglion	Strong		3F	Strong expression in cranial nerve ganglia V, VII, IX and X.
TS22: vagus ganglion	Strong		3F	Strong expression in cranial nerve ganglia V, VII, IX and X.

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present		3G	Expression in cranial nerve ganglia VII.

Structure	Level	Pattern	Image	Note
TS22: glossopharyngeal ganglion	Present		3H	Expression in cranial nerve ganglia IX.

Structure	Level	Pattern	Image	Note
TS22: ciliary ganglion	Strong		3I	Prominent expression in ciliary ganglion.

Structure	Level	Pattern	Image	Note
TS22: sphenopalatine ganglion	Strong		3J	Prominent expression in pterygopalatine ganglion.

Structure	Level	Pattern	Image	Note
TS17: genital swelling	Present	Regionally restricted	4A	Expression in regions bilaterally adjacent to cloacal membrane of genital folds.

Structure	Level	Pattern	Image	Note
TS19: genital swelling of genital tubercle	Strong	Regionally restricted	4B	Intense expression in emerging external genitalia.

Structure	Level	Pattern	Image	Note
TS19: genital swelling of genital tubercle	Strong	Regionally restricted	4C	Strong expression in genital swelling forming the genital tubercle. Bilateral expression pattern separated by urethral plate.

Structure	Level	Pattern	Image	Note
TS19: genital swelling of genital tubercle	Strong	Regionally restricted	4D	Strong expression in genital swelling forming the genital tubercle. Bilateral expression pattern separated by urethral plate.

Structure	Level	Pattern	Image	Note
TS19: genital swelling of genital tubercle	Strong	Regionally restricted	4E	Strong expression in genital swelling forming the genital tubercle. Bilateral expression pattern separated by urethral plate.

Structure	Level	Pattern	Image	Note
TS22: male external genitalia	Present		4F	Authors state that all examples shown are male embryos.

Structure	Level	Pattern	Image	Note
TS22: male external genitalia	Present		4G

Structure	Level	Pattern	Image	Note
TS22: male external genitalia	Present		4H

Structure	Level	Pattern	Image	Note
TS22: male external genitalia	Present	Regionally restricted	4I
TS22: scrotal swelling	Absent		4I	No expression in labioscrotal swelling.

Structure	Level	Pattern	Image	Note
TS22: male external genitalia	Present		4J

Structure	Level	Pattern	Image	Note
TS22: male external genitalia	Present		4K

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		5A

Structure	Level	Pattern	Image	Note
TS22: crus commune	Present		5B
TS22: posterior semicircular canal	Present		5B

Structure	Level	Pattern	Image	Note
TS22: lateral semicircular canal	Present		5C
TS22: saccule	Present		5C

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present		5D
TS22: metanephros	Present		5D

Structure	Level	Pattern	Image	Note
TS22: adrenal medulla	Present		5E

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Present		5F

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Present	Regionally restricted	5F inset	Authors report staining in lumen of metanephric tubule.

Structure	Level	Pattern	Image	Note
TS27: 3rd ventricle	Present		5G
TS27: lateral ventricle	Present		5G

Structure	Level	Pattern	Image	Note
TS27: 3rd ventricle choroid plexus	Present		5H

Structure	Level	Pattern	Image	Note
TS27: lateral ventricle choroid plexus	Present		5I

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		6A (left)
TS17: facial ganglion	Present		6A (left)
TS17: glossopharyngeal ganglion	Present		6A (left)
TS17: trigeminal ganglion	Present		6A (left)
TS17: vagus ganglion	Present		6A (left)
TS17: genital swelling	Present		6A (left)	Expression in external genitalia.

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Weak		6A (right)	Decreased expression in neural crest-derived cranial and dorsal root ganglion.
TS17: dorsal root ganglion	Weak		6A (right)	Decreased expression in neural crest-derived cranial and dorsal root ganglion.
TS17: genital swelling	Present		6A (right)	Expression in external genitalia.

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Present		6B (left)
TS19: dorsal root ganglion	Present		6B (left)
TS19: genital swelling of genital tubercle	Present		6B (left)	Expression in external genitalia.

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Weak		6B (right)	Decreased expression in neural crest-derived cranial and dorsal root ganglion.
TS19: dorsal root ganglion	Weak		6B (right)	Decreased expression in neural crest-derived cranial and dorsal root ganglion.
TS19: genital swelling of genital tubercle	Present		6B (right)	Expression in external genitalia not affected.

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present		6C (left)
TS20: dorsal root ganglion	Present		6C (left)
TS20: male external genitalia	Present		6C (left)

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Weak		6C (right)	Decreased expression in neural crest-derived cranial and dorsal root ganglion.
TS20: dorsal root ganglion	Weak		6C (right)	Decreased expression in neural crest-derived cranial and dorsal root ganglion.
TS20: male external genitalia	Present		6C (right)	Expression in external genitalia not affected.

Structure	Level	Pattern	Image	Note
TS21: male external genitalia	Present		6D (left)

Structure	Level	Pattern	Image	Note
TS21: male external genitalia	Present		6D (right)	Expression in external genitalia not affected.