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Gene Expression Data
Assay Details
Assay
Reference: J:275903 Shiura H, et al., Xist/Tsix expression dynamics during mouse peri-implantation development revealed by whole-mount 3D RNA-FISH. Sci Rep. 2019 Mar 6;9(1):3637
Assay type: RNA in situ
MGI Accession ID: MGI:6459735
Gene symbol: Xist
Gene name: inactive X specific transcripts
Probe: Xist probe6
Probe preparation: Antisense labelled with fluorescein RNA
Visualized with: Fluorescence
Assay notes: Single molecule fluorescence in situ hybridization (FISH) analysis. Probe was labeled with Green-dUTP.
Results
Specimen 1b: embryonic day 3.5 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1b merge , 1b Xist More than 80% of inner cell mass cells had one Xist cloud and one pinpoint Tsix signal per nucleus. Shown is a single cell with one Xist cloud and a single Tsix signal.
Specimen 1c: embryonic day 3.75 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1c merge , 1c Xist Nuclear cloud signals began to disappear and cells showing biallelic expression of Tsix began to appear. Shown is a single cell with no Xist and a single Tsix signal.
Specimen 1e: embryonic day 5.0 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS7: epiblast Present 1e merge , 1e Xist Shown is a single cell with one Xist pinpoint signal and two Tsix signals. 26% of the epiblast cells showed weak Xist expression as a pinpoint signal.
Specimen 1f: embryonic day 5.25 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS8: epiblast Present 1f Xist , 1f merge More than half of the cells (55%) had Xist signals with most of the cells showing dispersed Xist signals. Cells with Xist clouds began to appear. Shown is a single cell with one Xist dispersed signal and a single Tsix spot.
Specimen 1d,h: embryonic day 4.75 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS7: epiblast Present 1h , 1d Xist , 1d merge Encircled is a single cell with two Xist clouds and a single Tsix signal.
Specimen 1g,i: embryonic day 5.5 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS8: epiblast Present 1i , 1g merge , 1g Xist Encircled is a cell with two Xist clouds and a single Tsix signal.
Specimen 2c: embryonic day 5.0 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Gata6 (assay MGI:6459723).
Structure Level Pattern Image Note
TS7: epiblast Present 2c Most of the cells (88-98%) in the primitive endoderm/visceral endoderm lineage had one Xist cloud per nucleus.
Specimen 2d: embryonic day 4.25 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Cdx2 (assay MGI:6459726).
Structure Level Pattern Image Note
TS6: trophectoderm Present 2d Most of the Trophectoderm/Extraembryonic Ectoderm cells (70-95%) had one Xist cloud and a single Tsix spot.
Specimen 2e: embryonic day 4.5 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Cdx2 (assay MGI:6459726).
Structure Level Pattern Image Note
TS6: trophectoderm Present 2e , 2g , 2f Most of the Trophectoderm/Extraembryonic Ectoderm cells (70-95%) had one Xist cloud and a single Tsix spot.
Specimen S2a Lamp2: embryonic day 4.75 (more )
Note: Double labeled: green - Xist; magenta - Lamp2 (assay MGI:6459890).
Structure Level Pattern Image Note
TS7: epiblast Present S2a Lamp2
Specimen S2a Pgk1: embryonic day 4.75 (more )
Note: Double labeled: green - Xist; magenta - Pgk1 (assay MGI:6459891).
Structure Level Pattern Image Note
TS7: epiblast Present S2a Pgk1
Specimen S3b: embryonic day 5.25 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS8: epiblast Present S3b Xist , S3b Merge
Specimen S3c: embryonic day 5.5 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS8: epiblast Present S3c Xist , S3c Merge
Specimen S4a: embryonic day 5.5 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS8: epiblast Present S4a Xist , S4a Merge , S4a left
Specimen S4b: embryonic day 5.5 (more )
Note: Triple labeled: green - Xist; magenta - Tsix (assay MGI:6459736); blue - Pou5f1 (assay MGI:6459718).
Structure Level Pattern Image Note
TS7: epiblast Present S4b left , S4b Xist , S4b Merge
Specimen Matrix Figure 1a E4.0: embryonic day 4.0 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present Nuclear cloud signals began to disappear.
Specimen Matrix Figure 1a E4.25: embryonic day 4.25 (more )
Structure Level Pattern Image Note
TS6: epiblast Present Nuclear cloud signals began to disappear.
Specimen Matrix Figure 1a E4.5: embryonic day 4.5 (more )
Structure Level Pattern Image Note
TS6: epiblast Absent
Specimen Matrix Figure 1a E6.5: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: embryo Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory