Gene Expression Data
Assay Details
Assay
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Reference:
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J:291661
Hu Z, et al., Maternal factor NELFA drives a 2C-like state in mouse embryonic stem cells. Nat Cell Biol. 2020 Feb;22(2):175-186
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6455776
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Gene symbol:
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Nelfa
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Gene name:
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negative elongation factor complex member A, Whsc2
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Antibody:
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anti-Nelfa (#A301-910A)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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The authors described two antibodies but did not specify which was used to obtain these data; it is possible MGI:6455772 was used instead.
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Results
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Specimen
1c - oocyte:
postnatal
(more )
Specimen
1c - oocyte:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Female
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
|
1c - 1st
|
This is a maternally supplied factor.
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Specimen
1c - zygote:
embryonic day 0.5
(more )
Specimen
1c - zygote:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 0.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1c - 2 cell:
embryonic day 1.0
(more )
Specimen
1c - 2 cell:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 1.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1c - 4 cell:
embryonic day 2.0
(more )
Specimen
1c - 4 cell:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 2.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1c - blastocyst:
embryonic day 3.5
(more )
Specimen
1c - blastocyst:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
|