Antibody:	Anti-Pdx1
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Two Pdx1 antibodies were described: MGI:6455031 and MGI:6455032); either could have been used in this assay.

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium	Present	Regionally restricted	1B	Expression was detected in primary pancreatic progenitor cells in the dorsal pancreas. Expression overlapped with Sox9 immunoreactivity.
TS17: duodenum	Present		1B	Pdx1-expressing cells in the duodenum did not co-express Sox9.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Present	Regionally restricted	1D	Expression was detected in primary pancreatic progenitor cells in the dorsal pancreas. Expression overlapped with Sox9 immunoreactivity.

Structure	Level	Pattern	Image	Note
TS22: pancreatic duct	Weak	Regionally restricted	1E	Expression at lower levels was detected in the secondary pancreatic progenitor cells along the ducts. The secondary pancreatic progenitor cells co-express Sox9.
TS22: pancreas	Present	Regionally restricted	1E	Intensely Pdx1-stained differentiated cells did not co-express Sox9.