Adipor2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:165154 Cikos S, et al., Expression of adiponectin receptors and effects of adiponectin isoforms in mouse preimplantation embryos. Hum Reprod. 2010 Sep;25(9):2247-55
Assay type:	RT-PCR
MGI Accession ID:	MGI:6450124
Gene symbol:	Adipor2
Gene name:	adiponectin receptor 2

Probe:	Adipor2-pC, Adipor2-pD
Probe preparation:	labelled with SYBR green
Assay notes:	This assay is a quantitative RT-PCR. Actb was used as the standard.

Results

Image: 2_AdipoR2

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	258 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Oc	P w 4-5	TS28: secondary oocyte	Present	Not Specified; total RNA	ICR		Female	unfertilized oocytes at metaphase II
4c	E2.0 (b)	TS3: 4-cell stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified	4-cell embryo
8c	E2.5 (b)	TS3: 8-cell stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified	8-cell embryo
Mo	E3.0 (b)	TS4: morula-stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified	morula
Bl	E3.5 (b)	TS4: blastocyst-stage conceptus	Present	Not Specified; total RNA	ICR		Not Specified	blastocyst

Notes:
(a) Expression was significantly lower in 4-cell embryos and 8- to 16-cell embryos than in oocytes, and it increased significantly above the oocyte level in morulas and blastocysts.
(b) Age assigned by curator based on morphological criteria supplied by authors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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