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Gene Expression Data
Assay Details
Assay
Reference: J:100667 Amireault P, et al., Serotonin and its antidepressant-sensitive transport in mouse cumulus-oocyte complexes and early embryos. Biol Reprod. 2005 Aug;73(2):358-65
Assay type: RT-PCR
MGI Accession ID: MGI:6450070
Gene symbol: Tph1
Gene name: tryptophan hydroxylase 1
Probe: Tph1 primer set 2
Visualized with: Ethidium bromide
Assay notes: Nested RT-PCR was used to obtain these results; the outer primer set was MGI:6450049
Results Image: 2A Tph
 Sample Information Bands Other Sample Information
Lane AgeStructure 389 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
1 P w 3-4 TS28: ovary Present Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Female total ovary
2 P w 3-4 TS28: cumulus oophorus Present Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Female isolated cumulus cells
3 P w 3-4 TS28: primary oocyte Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Female germinal vesicle (GV) stage oocytes
4 P w 3-4 TS28: secondary oocyte Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Female metaphase II (MII) stage oocytes
5 E0.5 (a) TS1: 1-cell stage embryo Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Not Specified
6 E1.0 (a) TS2: 2-cell stage embryo Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Not Specified
7 E2.0 (a) TS3: 4-cell stage embryo Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Not Specified
8 E2.5 (a) TS3: 8-cell stage embryo Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Not Specified
9 E3.0 (a) TS3: compacted morula Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Not Specified
10 E3.5 (a) TS4: blastocyst Absent Not Specified; poly-A+ RNA (C57BL/6 x C3H)F1 Not Specified
11 Control
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory