Gene Expression Data
Assay Details
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Assay
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Reference:
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J:290740
Brachet C, et al., Truncating RAX Mutations: Anophthalmia, Hypopituitarism, Diabetes Insipidus, and Cleft Palate in Mice and Men. J Clin Endocrinol Metab. 2019 Jul 1;104(7):2925-2930
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6448085
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Gene symbol:
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Lhx3
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Gene name:
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LIM homeobox protein 3
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Antibody:
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Lhx3 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2K:
embryonic day 10.0
(more  )
Specimen
2K:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2L:
embryonic day 11.5
(more )
Specimen
2L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2M:
embryonic day 12.5
(more )
Specimen
2M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2O:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Raxtm1Mjam/Raxtm1Mjam
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: Rathke's pouch |
Present |
Regionally restricted |
2O
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Authors report normal Rathke pouch invagination lining roof of oral cavity in mutant embryos.
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Specimen
2P:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Raxtm1Mjam/Raxtm1Mjam
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: Rathke's pouch |
Present |
Regionally restricted |
2P
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Defects in Rathke pouch invagination, with expression lining roof of oral cavity in mutant embryos, little changed from E10 compared with controls.
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Specimen
2Q:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Raxtm1Mjam/Raxtm1Mjam
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools