Gene Expression Data
Assay Details
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Assay
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Reference:
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J:290315
Cheong A, et al., Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development. 2020 May 26;147(10):dev188714
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6445619
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Gene symbol:
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Cdc25c
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Gene name:
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cell division cycle 25C
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Antibody:
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anti-Cdc25c (phospho-S216; orb159479)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 647
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Assay notes:
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The antibody used in this assay recognizes the phosphorylated form of the protein.
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Results
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Specimen
7A:
embryonic day 7.5
(more  )
Note: Littermate control; embryo is either +/+ or +/-. Double labeled: red - phosphorylated Cdc25c; green - Cdh1 (assay MGI:6445567).
Specimen
7A:
(close  )
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Genetic Background:
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C57BL/6N
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Littermate control; embryo is either +/+ or +/-. Double labeled: red - phosphorylated Cdc25c; green - Cdh1 (assay MGI:6445567).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: conceptus |
Present |
Single cells |
7A
,
7A''
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The phosphorylated protein indicates cells at the G2/M checkpoint.
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Specimen
7B:
(close )
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Genetic Background:
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C57BL/6N-Mrpl3em1(IMPC)J/Mmucd
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Mutant Allele(s):
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Mrpl3em1(IMPC)J/Mrpl3em1(IMPC)J
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - phosphorylated Cdc25c; green - Cdh1 (assay MGI:6445567).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: conceptus |
Present |
Single cells |
7B
,
7B''
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The phosphorylated protein indicates cells at the G2/M checkpoint. There were significantly more of these cells in the mutant compared to wild type. Mutants appear to stall just prior to the onset of gastrulation.
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Specimen
not shown:
(close )
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Genetic Background:
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C57BL/6N-Atm1Brd Mrpl22tm1b(KOMP)Mbp/JMmucd
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Mutant Allele(s):
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Mrpl22tm1b(KOMP)Mbp/Mrpl22tm1b(KOMP)Mbp
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: conceptus |
Present |
Single cells |
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These results were reported in Fig. 7E. The phosphorylated protein indicates cells at the G2/M checkpoint. There were significantly more of these cells in the mutant compared to wild type. Mutants appear to stall just prior to the onset of gastrulation.
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Specimen
not shown:
(close )
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Genetic Background:
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C57BL/6N-Mrps22tm1.1(KOMP)Vlcg/JMmucd
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Mutant Allele(s):
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Mrps22tm1.1(KOMP)Vlcg/Mrps22tm1.1(KOMP)Vlcg
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: conceptus |
Present |
Single cells |
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These results were reported in Fig. 7E. The phosphorylated protein indicates cells at the G2/M checkpoint. There were significantly more of these cells in the mutant compared to wild type. Mutants appear to stall just prior to the onset of gastrulation.
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Specimen
7C:
embryonic day 5.5
(more )
Note: Early egg-cylinder stage embryo. Double labeled: red - phosphorylated Cdc25c; green - Cdh1 (assay MGI:6445567).
Specimen
7C:
(close )
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Genetic Background:
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C57BL/6N
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early egg-cylinder stage embryo. Double labeled: red - phosphorylated Cdc25c; green - Cdh1 (assay MGI:6445567).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS7: conceptus |
Present |
Single cells |
7C''
,
7C
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The phosphorylated protein indicates cells at the G2/M checkpoint.
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Analysis Tools