Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143023
Coumailleau P, et al., Sim1 and Sim2 expression during chick and mouse limb development. Int J Dev Biol. 2009;53(1):149-57
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6437446
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Gene symbol:
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Sim1
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Gene name:
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single-minded family bHLH transcription factor 1
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Probe:
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Sim1 probe7
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 10.0
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: dermomyotome |
Present |
Regionally restricted |
5A
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Expression in lateral region of dermomyotome at forelimb limb level.
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| TS16: forelimb bud mesenchyme |
Weak |
Regionally restricted |
5A
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Faint expression in subset of somitic cells migrating to limb buds - muscle progenitor cells.
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Specimen
5C:
embryonic day 10.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
Regionally restricted |
5C
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Expression in lateral region of dermomyotome at hindlimb level.
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| TS17: hindlimb bud mesenchyme |
Weak |
Regionally restricted |
5C
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Faint expression in subset of somitic cells migrating to limb buds - muscle progenitor cells.
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| TS17: mesonephros |
Present |
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5C
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Specimen
5F:
embryonic day 10.5
(more )
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Present |
Regionally restricted |
5F
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Expression in lateral somite. Authors report expression domain defined a lateral compartment of somites and of somitic-derived tissues, including lateral regions of sclerotome and dermomyotome, and its derivatives, dermatome and myotome.
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