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Gene Expression Data
Assay Details
Assay
Reference: J:286601 Jiang Y, et al., CXXC finger protein 1-mediated histone H3 lysine-4 trimethylation is essential for proper meiotic crossover formation in mice. Development. 2020 Mar 17;147(6):dev183764
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6435212
Gene symbol: Dmc1
Gene name: DNA meiotic recombinase 1
Antibody: anti-Dmc1 (13714-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 4C-WT-early zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Dmc1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C WT EZ Expression is present in foci.

Specimen 4C-WT-late zygotene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Dmc1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C WT LZ Expression is present in foci.

Specimen 4C-WT-early pachytene: postnatal adult (more )
Note: Wild-type littermate control; it retains at least one floxed allele. Double labeled: red - Dmc1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C WT EP Expression is present in foci.

Specimen 4C-mut-early zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - Dmc1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C mut EZ Expression is present in foci; the number of these foci in the mutant is comparable to the number in wild type.

Specimen 4C-mut-late zygotene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - Dmc1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C mut LZ Expression is present in foci; the number of these foci in the mutant is comparable to the number in wild type.

Specimen 4C-mut-early pachytene: postnatal adult; Cxxc1tm1.1Liwa/Cxxc1tm1.2Liwa, Stra8em1(GFP/cre)Smoc/Stra8+ (more )
Note: Conditional mutant. Double labeled: red - Dmc1; green - Sycp3 (assay MGI:6435158)
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C mut EP Expression is present in foci; the number of these foci in the mutant is increased compared to the number in wild type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory