Map1lc3a Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:285853 Zhang S, et al., WD40 repeat and FYVE domain containing 3 is essential for cardiac development. Cardiovasc Res. 2019 Jul 1;115(8):1320-1331
Assay type:	Western blot
MGI Accession ID:	MGI:6433805
Gene symbol:	Map1lc3a
Gene name:	microtubule-associated protein 1 light chain 3 alpha

Antibody:	anti-LC3
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Antibody specificity was not detailed; both family members have been annotated.

Results

Image: 6A LC3

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	16 kDa (a)	14 kDa (b)	Genetic Background	Mutant Allele(s)	Sex
WT	E14.5	TS22: heart	Present	Present	involves: C57BL/6 * FVB/NJ		Not Specified
WT	E14.5	TS22: heart	Present	Present	involves: C57BL/6 * FVB/NJ		Not Specified
WT	E14.5	TS22: heart	Present	Present	involves: C57BL/6 * FVB/NJ		Not Specified
Wdfy3^PB/PB	E14.5	TS22: heart	Present	Present	involves: C57BL/6 * FVB/NJ	Wdfy3^{Tn(pb-Act-RFP)1.1Zhu}/Wdfy3^{Tn(pb-Act-RFP)1.1Zhu}	Not Specified
Wdfy3^PB/PB	E14.5	TS22: heart	Present	Present	involves: C57BL/6 * FVB/NJ	Wdfy3^{Tn(pb-Act-RFP)1.1Zhu}/Wdfy3^{Tn(pb-Act-RFP)1.1Zhu}	Not Specified
Wdfy3^PB/PB	E14.5	TS22: heart	Present	Present	involves: C57BL/6 * FVB/NJ	Wdfy3^{Tn(pb-Act-RFP)1.1Zhu}/Wdfy3^{Tn(pb-Act-RFP)1.1Zhu}	Not Specified

Notes:
(a) This band represents the LC3 II isoform. There is no signficant difference in expression levels between mutants and wild type.
(b) This band represents the LC3 I isoform. It was not discussed, but it appears there is no signficant difference in expression levels between mutants and wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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