Antibody:	5-HT-2A-R antibody (C-terminus)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The authors did not specify which antibody was used. It is possible a N-terminal anitbody was used in the assay: 5-HT-2A-R antibody (N-terminus) (MGI:6432839).

Structure	Level	Pattern	Image	Note
TS13: 1st branchial arch mesenchyme	Present		1A
TS13: future brain neural fold	Present		1A
TS13: future spinal cord	Present		1A
TS13: notochord	Present		1A
TS13: gut endoderm	Present	Regionally restricted	1A	Expression is in the gut endothelium.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present		1B
TS14: future midbrain	Present		1B
TS14: future spinal cord	Present		1B
TS14: heart	Present		1B
TS14: prosencephalon	Present		1B
TS14: somite	Present		1B
TS14: head mesenchyme	Present	Regionally restricted	1B	Expression is in craniofacial mesenchyme.
TS14: gut	Present	Regionally restricted	1B	Expression is in the gut endothelium.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present		1C
TS14: future midbrain	Present		1C
TS14: future spinal cord	Present		1C
TS14: heart	Present		1C
TS14: prosencephalon	Present		1C
TS14: somite	Present		1C
TS14: head mesenchyme	Present	Regionally restricted	1C	Expression is in craniofacial mesenchyme.
TS14: gut	Present	Regionally restricted	1C	Expression is in the gut endothelium.

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme	Present	Regionally restricted	1D	Expression is in the mesenchyme layer adjacent to the forebrain.
TS16: head surface ectoderm	Present	Regionally restricted	1D	Expression is in the epithelial layer adjacent to the forebrain.

Structure	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	Present		1E
TS16: endocardial cushion	Present		1E
TS16: gut	Present		1E
TS16: somite	Present		1E

Structure	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	Present		1F
TS16: endocardial cushion	Present		1F
TS16: gut	Present		1F
TS16: somite	Present		1F

Structure	Level	Pattern	Image	Note
TS17: atrioventricular endocardial cushion	Present		1G
TS17: cardiac muscle tissue	Present		1G
TS17: outflow tract endocardial cushion	Present		1G

Structure	Level	Pattern	Image	Note
TS19: myelencephalon floor plate	Present		1H
TS19: notochord	Present		1H

Structure	Level	Pattern	Image	Note
TS19: fronto-nasal process ectoderm	Present		1I
TS19: fronto-nasal process mesenchyme	Present		1I

Structure	Level	Pattern	Image	Note
TS19: atrioventricular endocardial cushion	Present
TS19: cardiac muscle tissue	Present
TS19: outflow tract endocardial cushion	Present

Structure	Level	Pattern	Image	Note
TS21: striated muscle tissue	Present	Regionally restricted	2A	Expression is in the craniofacial region.

Structure	Level	Pattern	Image	Note
TS23: nasal septum cartilage	Present		2C

Structure	Level	Pattern	Image	Note
TS23: Meckel's cartilage	Present		2D
TS23: tooth	Present		2D

Structure	Level	Pattern	Image	Note
TS23: tongue muscle	Present		2E

Structure	Level	Pattern	Image	Note
TS23: vibrissa follicle	Present		2F

Structure	Level	Pattern	Image	Note
TS25: chondrocranium	Present		2G

Structure	Level	Pattern	Image	Note
TS25: vibrissa follicle	Present		2H