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Gene Expression Data
Assay Details
Assay
Reference: J:285969 Liu H, et al., SCRE serves as a unique synaptonemal complex fastener and is essential for progression of meiosis prophase I in mice. Nucleic Acids Res. 2019 Jun 20;47(11):5670-5683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6431480
Gene symbol: Rpa2
Gene name: replication protein A2
Antibody: anti-RPA32/RPA2 [EPR2877Y] (ab76420)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S6a +/+ leptotene: postnatal day 35 (more )
Note: Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a +/+ leptotene Expression is present in recombination nodules.
Specimen S6a +/+ zygotene: postnatal day 35 (more )
Note: Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a +/+ zygotene Expression is present in recombination nodules.
Specimen S6a +/+ pachytene: postnatal day 35 (more )
Note: Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a +/+ pachytene Expression is present in recombination nodules.
Specimen S6a -/- leptotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a -/- leptotene Expression is present in recombination nodules; the number of foci is similar in mutants and wild type.
Specimen S6a -/- zygotene: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a -/- zygotene Expression is present in recombination nodules; the number of foci is similar in mutants and wild type.
Specimen S6a -/- pachytene-like: postnatal day 35; 1700028K03Rikem1Jzc/1700028K03Rikem1Jzc (more )
Note: Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6431450).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a -/- pachytene-like Expression is present in recombination nodules; the number of foci is increased in mutants compared to wild type, suggesting a slower progression through meiosis.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory