Gene Expression Data
Assay Details
Assay
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Reference:
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J:285730
Major AT, et al., Dynamic paraspeckle component localisation during spermatogenesis. Reproduction. 2019 Sep;158(3):267-280
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6430464
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Gene symbol:
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Pspc1
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Gene name:
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paraspeckle protein 1
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Antibody:
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anti-PSPC1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2A E12.5:
embryonic day 12.5
(more )
Specimen
2A E12.5:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
2A E14.5:
embryonic day 14.5
(more )
Specimen
2A E14.5:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 14.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
2A E16.5:
embryonic day 16.5
(more )
Specimen
2A E16.5:
(close )
Genetic Background:
|
Swiss
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
2B E12.5:
embryonic day 12.5
(more )
Specimen
2B E12.5:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 12.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: germ cell of testis |
|
Present |
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2B E12.5
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The protein is localized in the germ cell nuclei.
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TS20: Sertoli cell |
Sertoli cell |
Absent |
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2B E12.5
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The protein is not present in the somatic cells of the testis.
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Specimen
2B E14.5:
embryonic day 14.5
(more )
Specimen
2B E14.5:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: germ cell of testis |
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Present |
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2B E14.5
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The protein is localized in the germ cell nuclei.
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TS22: Sertoli cell |
Sertoli cell |
Absent |
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2B E14.5
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The protein is not present in the somatic cells of the testis.
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Specimen
2B E16.5:
embryonic day 16.5
(more )
Specimen
2B E16.5:
(close )
Genetic Background:
|
Swiss
|
Age:
|
embryonic day 16.5
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Sex:
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Male
|
Type:
|
section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: germ cell of testis |
|
Present |
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2B E16.5
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The protein is localized in the germ cell nuclei.
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TS24: Sertoli cell |
Sertoli cell |
Absent |
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2B E16.5
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The protein is not present in the somatic cells of the testis.
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Specimen
3A Day 0:
postnatal day 0
(more )
Specimen
3A Day 0:
(close )
Genetic Background:
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involves: C57BL/6 * CBA * Swiss
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Age:
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postnatal day 0
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
3A Day 6:
postnatal day 6
(more )
Specimen
3A Day 6:
(close )
Genetic Background:
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involves: C57BL/6 * CBA * Swiss
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Age:
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postnatal day 6
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Sex:
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Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
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Specimen
3A Day 10:
postnatal day 10
(more )
Specimen
3A Day 10:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA * Swiss
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Age:
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postnatal day 10
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Sex:
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Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
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Specimen
3A Day 15:
postnatal day 15
(more )
Specimen
3A Day 15:
(close )
Genetic Background:
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involves: C57BL/6 * CBA * Swiss
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Age:
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postnatal day 15
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Sex:
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Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: spermatogonium |
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Present |
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3A Day 15
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Expression is seen in the nuclei.
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TS28: Sertoli cell |
Sertoli cell |
Present |
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3A Day 15
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Expression is seen in the nuclei.
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TS28: spermatocyte |
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Present |
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3A Day 15
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Expression is seen in the nuclei; it is excluded from the XY body.
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Specimen
3A Day 26:
postnatal day 26
(more )
Specimen
3A Day 26:
(close )
Genetic Background:
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involves: C57BL/6 * CBA * Swiss
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Age:
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postnatal day 26
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
3A Adult:
postnatal adult
(more )
Specimen
3A Adult:
(close )
Genetic Background:
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involves: C57BL/6 * CBA * Swiss
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
|
Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
|
Present |
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3B Adult--top
,
3B Adult--left zoom
,
3B Adult--lower
,
3B Adult--center
,
3B Adult--right zoom
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Expression is mainly seen in the nuclei; it is excluded from the XY body. At stage XII, expression was distributed in the cytoplasm.
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TS28: spermatid |
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Present |
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3B Adult--center
,
3B Adult--top
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Expression is seen in round spermatids.
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TS28: Sertoli cell |
Sertoli cell |
Present |
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3B Adult--lower
,
3B Adult--top
,
3B Adult--center
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TS28: Leydig cell |
Leydig cell |
Absent |
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3B Adult--top
,
3B Adult--center
,
3B Adult--lower
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TS28: peritubular myoid cell |
peritubular myoid cell |
Absent |
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3B Adult--top
,
3B Adult--center
,
3B Adult--lower
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