Hoxa1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:173839 Bertrand N, et al., Hox genes define distinct progenitor sub-domains within the second heart field. Dev Biol. 2011 May 15;353(2):266-74
Assay type:	RNA in situ
MGI Accession ID:	MGI:6430415
Gene symbol:	Hoxa1
Gene name:	homeobox A1

Probe:	Hoxa1 probe11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

S1J ©

Specimen S1J: embryonic day 7.25 (more

)

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		S1J	Transcription initiates earlier than Hoxa1 and Hoxa3.

S1K ©

Specimen S1K: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	S1K	Authors report expansion of expression in anterior lateral mesoderm.

S1L ©

Specimen S1L: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: embryo mesoderm	Present	Regionally restricted	S1L	Authors report expansion of expression in anterior lateral mesoderm.

1D ©

Specimen 1D: embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo mesoderm	Present	Regionally restricted	1D	Most anterior border of expression near cardiac crescent.

2C ©

Specimen 2C: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2C	Expression in ventral and lateral foregut endoderm.
TS13: secondary heart field	Present	Regionally restricted	2C	Expression in splanchnic mesoderm only in most caudal margin of anterior domain of second heart field (AHF).

2C1 ©

Specimen 2C1: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2C1	Expression in anterior foregut endoderm.

2C2 ©

Specimen 2C2: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2C2	Expression in anterior foregut endoderm.
TS13: secondary heart field	Present	Regionally restricted	2C2	Expression in splanchnic mesoderm only in most caudal margin of anterior domain of second heart field (AHF).

2C3 ©

Specimen 2C3: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present	Regionally restricted	2C3
TS13: secondary heart field	Present	Regionally restricted	2C3	Expression in posterior region of second heart field.
TS13: endocardial tube	Absent		2C3	No expression in heart tube.

S6D ©

Specimen S6D: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present		S6D

S6D inset ©

Specimen S6D inset: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Present		S6D inset

S6E ©

Specimen S6E: embryonic day 8.5; Aldh1a2^tm1Ipc/Aldh1a2^tm1Ipc (more

)

Structure	Level	Pattern	Image	Note
TS13: splanchnopleure	Weak		S6E	Expression downregulated in splanchnic mesoderm in mutant embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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