Antibody:	anti-B tubulin isotype III (T8660)
Detection system:	Secondary antibody coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: peripheral nervous system	Present	Single cells	4A	Neurons are clearly recognisable in the gut mesenchyme.
TS19: gonad	Absent		4A

Structure	Level	Pattern	Image	Note
TS19: peripheral nervous system	Present	Single cells	4B	Neurons are clearly recognisable in the gut mesenchyme.
TS19: gonad	Absent		4B

Structure	Level	Pattern	Image	Note
TS17: gonad primordium	Absent		5A , 5C	No expression is observed in the gonad or adjacent tissue.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Single cells	5D	Neurons are clearly recognisable in the future brain.
TS13: future spinal cord	Present	Single cells	5D	Neurons are starting to appear in the neural tube. There is no expression in the ganglia.
TS13: gut mesenchyme	Absent		5D
TS13: gut mesentery	Absent		5D
TS13: trunk mesenchyme	Absent		5D	No expression is observed in the peri-aortic region nor in the prospective gonad.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Single cells	5E	Neurons are clearly recognizable in the future brain.
TS15: future spinal cord	Present	Single cells	5E	Neurons are clearly recognizable in the neural tube.
TS15: ganglion	Present	Single cells	5E	Neuronal staining is starting to appear in the ganglia.
TS15: peripheral nervous system	Present	Single cells	5E	There is some signal observed in the gut mesenchyme. None is observed in the peri-aortic region or in the mesentery.
TS15: urogenital ridge	Absent		5E	No expression is observed in the prospective gonad.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong		5F	Expression is present in many cells.
TS17: future spinal cord	Strong		5F	Expression is present in many cells.
TS17: ganglion	Strong		5F	Expression is present in many cells.
TS17: peripheral nervous system	Present	Single cells	5F	Neurons are clearly recognisable in the gut mesenchyme; they are starting to appear in the peri-aortic region. Presence of neurons in the mesentery was not observed but cannot be excluded.
TS17: gonad primordium	Absent		5F

Structure	Level	Pattern	Image	Note
TS19: brain	Strong		5G	Expression is present in many cells.
TS19: future spinal cord	Strong		5G	Expression is present in many cells.
TS19: ganglion	Strong		5G	Expression is present in many cells.
TS19: peripheral nervous system	Present	Single cells	5G	Neurons are clearly recognisable in the gut mesenchyme and the peri-aortic region. Presence of neurons in the mesentery was not observed but cannot be excluded.
TS19: gonad	Absent		5G