Antibody:	anti-NHS
Detection system:	Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Strong		9B	Strong expression in eye. Expression in lens and retina throughout development.
TS20: retina	Strong		9B	Strong expression in eye. Expression in lens and retina throughout development.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle epithelium	Present	Regionally restricted	9E	Authors report highest subcellular levels of expression at apical side, then basal side, with lower levels at lateral walls of epithelial cells.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong		9F	Strong expression in neural epithelium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	9G	Authors report highest subcellular levels of expression at apical side, then basal side, with lower levesls at lateral walls of epithelial cells

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	9H	Apical and basal concentration of expression, as well as localization in lateral walls of epithelium.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Strong	Regionally restricted	9I	Strong expression in blood vessel endothelium.

Structure	Level	Pattern	Image	Note
TS20: epidermis	Present		9J	Expression in surface ectoderm.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	9K	Authors report highest subcellular levels of expression at apical side, then basal side, with lower levesl at lateral walls of epithelial cells

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Strong		9L

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	9M	Highest concentration of expression in apical aspects of epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	9N	Authors report highest subcellular levels of expression at apical side, then basal side, with lower levesl at lateral walls of epithelial cells

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	9O	Authors report highest subcellular levels of expression at apical side, then basal side, with lower levesl at lateral walls of epithelial cells

Structure	Level	Pattern	Image	Note
TS26: molar enamel organ	Present		9P

Structure	Level	Pattern	Image	Note
TS26: alveolus epithelium	Strong			At E18.5, strongest expression in bronchiolar epithelium and alveolar epithelium.
TS26: bronchiole epithelium	Strong			At E18.5, strongest expression in bronchiolar epithelium and alveolar epithelium.
TS26: epidermis	Present	Regionally restricted		Expression in basal cells of epidermis.
TS26: hair follicle	Present
TS26: brain ventricular layer	Strong			Strong expression in ependymal layer in brain.
TS26: choroid plexus	Strong
TS26: intestine epithelium	Ambiguous			Low to undetectable levels of expression in intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS27: brain blood vessel	Strong	Regionally restricted	9Q	Authors report high levels of expression in vascular endothelium throughout development.

Structure	Level	Pattern	Image	Note
TS27: brain ependyma	Strong		9R
TS27: lateral ventricle choroid plexus	Strong		9R

Structure	Level	Pattern	Image	Note
TS27: brain ependyma	Strong		9S

Structure	Level	Pattern	Image	Note
TS27: meninges	Strong		9T