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Gene Expression Data
Assay Details
Assay
Reference: J:266583 Baumgartner M, et al., Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells. Development. 2018 Aug 28;145(17):dev166322
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6400916
Gene symbol: Aurkb
Gene name: aurora kinase B
Results
Specimen 5D control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Aurkb; magenta - Pax6 (assay MGI:6400919).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 5D E12 ctrl The staining pattern corresponds to specific phases within mitosis.
Specimen 5D mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Aurkb; magenta - Pax6 (assay MGI:6400919).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 5D E12 mut A significantly higher percentage of mitotic Aurkb+/Pax6+ radial glial cells were in prometaphase+metaphase in the E12 mutant pallium than in the control. Prophase, anaphase, and telophase cells were not significantly different compared to control.
Specimen 6C: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Aurkb; magenta - Trp53 (assay MGI:6400915).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 6C Quantification of cells with G2-specific Aurkb staining in the E12 mutant pallium revealed that 14.4% of Trp53-upregulating cells were in G2 phase, a significantly lower percentage than expected.
Specimen 6E: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Triple labeled: magenta - Aurkb; green - Casp3 (assay MGI:6400589); yellow - TUNEL.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 6E Virtually no cleaved Casp3+ cells were positively stained with Aurkb (2.1%).
Specimen S4A: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: neocortex Present S4A Immunofluorescence showed mitosis phase-specific staining patterns in cells in the pallium.
Specimen S4B control: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11+, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Aurkb; magenta - Eomes (assay MGI:6400773).
Structure Level Pattern Image Note
TS20: neocortex Present S4B ctrl The staining pattern corresponds to specific phases within mitosis.
Specimen S4B mutant: embryonic day 12.5; Rnu11tm1.1Rank/Rnu11tm1.1Rank, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - Aurkb; magenta - Eomes (assay MGI:6400773).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted S4B mut There were no significant differences in the fractions of mitotic intermediate progenitor cells in the mutant pallium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory