Antibody:	anti-myosin VIIa (#25-6790)
Detection system:	Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Regionally restricted	1B , 1C	Cytoplasmic expression in cochleovestibular ganglion (CVG). Expression in delaminated CVG neurons. No expression in geniculate ganglion.
TS15: otic pit epithelium	Present	Single cells	1B , 1C	Expression in a few cells within otic epithelium adjacent to CVG, likely neuroblasts on the verge of delamination.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Single cells	1C inset	Co-expression with Isl1 in cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Regionally restricted	1F , 1E	Cytoplasmic expression in cochleovestibular ganglion. No expression in geniculate ganglion.

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Regionally restricted	1F inset	Co-expression with Tubb3 in cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	2B , 2C	Cytoplasmic expression in subset of neurons around outer edge of cochleovestibular ganglion; No expression in central population of cochleovestibular ganglion neurons, likely post-mitotic neurons.
TS17: facial ganglion	Absent		2B , 2C	No expression in geniculate ganglion.
TS17: otocyst epithelium	Present	Single cells	2C , 2B	Expression in a few cells in otic epithelium.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	2C inset	Co-expression with Isl1 in cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Punctate	2E , 2F	Cytoplasmic expression in subset of neurons around outer edge of cochleovestibular ganglion; No expression in central population of cochleovestibular ganglion neurons, likely post-mitotic neurons.
TS17: facial ganglion	Absent		2E , 2F	No expression in geniculate ganglion.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	2F inset	Co-expression with Tubb3 in cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	2H , 2I	Cytoplasmic expression in subset of neurons around outer edge of cochleovestibular ganglion; No expression in central population of cochleovestibular ganglion neurons, likely post-mitotic neurons.
TS19: facial ganglion	Absent		2I , 2H	No expression in geniculate ganglion.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	2I inset	Co-expression with Isl1 in cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	2K , 2L	Cytoplasmic expression in subset of neurons around outer edge of cochleovestibular ganglion; No expression in central population of cochleovestibular ganglion neurons, likely post-mitotic neurons.
TS19: facial ganglion	Absent		2K , 2L	No expression in geniculate ganglion.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	2L inset	Co-expression with Tubb3 in cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS20: vestibular ganglion	Present	Regionally restricted	3C , 3B	Cytoplasmic expression in most neurons in vestibular ganglion. Expression dowregulated in subset of neurons.
TS20: cochlear ganglion	Absent		3B , 3C
TS20: vestibular component epithelium	Present	Regionally restricted	3B , 3C	Expression in cells in otic epithelium not co-expressing Tubb3, thus likely presumptive vestibular hair cells.

Structure	Level	Pattern	Image	Note
TS20: vestibular ganglion	Present	Regionally restricted	3C inset	Co-expression with Tubb3 in vestibular ganglion.

Structure	Level	Pattern	Image	Note
TS21: cochlear ganglion	Absent		3E , 3F
TS21: vestibular ganglion	Absent		3E , 3F
TS21: vestibular component epithelium	Present	Regionally restricted	3E , 3F	Clear cytoplasmic expression in vestibular hair cells.