Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS15: nasal process	Strong		2A	High expression in facial process.
TS15: cranial preganglion	Present		2A
TS15: limb bud	Present		2A
TS15: central nervous system	Weak	Regionally restricted	2A	Faint expression in certain areas of central nervous system.
TS15: somite	Weak		2A

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Strong		2A inset
TS15: otic pit	Present		2A inset	Authors report expression in otic placode.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	2B	Expression in facial mesenchyme recedes to ventro-rostral region including nasal invagination.
TS17: nasal pit	Present		2B	Expression in facial mesenchyme recedes to ventro-rostral region including nasal invagination.
TS17: optic cup	Present		2B
TS17: sensory organ	Present		2B	Expresison in all developing sensory organs.
TS17: telencephalon	Present	Regionally restricted	2B	Faint expression in basal telencephalon in region corresponding to ganglionic eminence.
TS17: forebrain-midbrain boundary region	Present	Regionally restricted	2B	Expression in ventricular zone at border between diencephalon and mesencephalon.
TS17: cranial ganglion	Strong		2B
TS17: dorsal root ganglion	Strong		2B
TS17: enteric nervous system	Present		2B	Significant expression in sympathetic chain and enteric nervous system.
TS17: sympathetic nerve trunk	Present		2B	Significant expression in sympathetic chain and enteric nervous system.
TS17: limb bud	Present	Regionally restricted	2B	Expression restricted to base of limb buds.
TS17: myotome	Weak		2B
TS17: heart	Absent		2B
TS17: liver	Absent		2B

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	2C	Expression in facial mesenchyme recedes to ventro-rostral region including nasal invagination.
TS19: nasal pit	Present		2C	Expression in facial mesenchyme recedes to ventro-rostral region including nasal invagination.
TS19: hindbrain	Present	Regionally restricted	2C	Expression in pontine region of hindbrain.
TS19: cranial ganglion	Strong		2C
TS19: dorsal root ganglion	Strong		2C
TS19: myotome	Present		2C
TS19: limb bud	Present	Regionally restricted	2C	Expression restricted to base of limb buds.
TS19: heart	Ambiguous	Regionally restricted	2C	Expression consistently absent in heart, liver and adipose tissue.
TS19: liver	Ambiguous	Regionally restricted	2C	Expression consistently absent in heart, liver and adipose tissue.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present		2E
TS22: olfactory lobe	Present	Regionally restricted	2E	Expression in lateral olfactory tract.
TS22: ganglionic eminence	Present	Regionally restricted	2E	In ganglionic eminence, expression in ventricular, subventricular and mantle zones.
TS22: central nervous system	Strong	Regionally restricted	2E	Increased expression in distinct regions of central nervous system.
TS22: forebrain-midbrain boundary region	Present		2E
TS22: medulla oblongata	Present	Regionally restricted	2E	Expression in some nuclei of pons and medulla oblongata.
TS22: pons	Present	Regionally restricted	2E	Expression in some nuclei of pons and medulla oblongata.
TS22: enteric nervous system	Strong		2E
TS22: peripheral nervous system	Present	Regionally restricted	2E	Expression fades in other part of peripheral nervous system.
TS22: metanephros	Present	Regionally restricted	2E	From E14.5, expression in kidney restricted to glomeruli and proximal tubules.

Structure	Level	Pattern	Image	Note
TS24: corpus striatum	Strong	Widespread	2F	Prominent expression in all layers of striatum.
TS24: brain ventricular layer	Strong		2F
TS24: cerebellum	Present		2F
TS24: diaphragm	Present		2F
TS24: intercostal muscle	Present		2F
TS24: hyoid	Strong		2F	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: limb cartilage condensation	Strong		2F	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: rib cartilage	Strong		2F	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: sensory organ cartilage	Strong		2F	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: thyroid cartilage	Strong		2F	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: vertebral cartilage condensation	Strong		2F	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: metanephros	Present	Regionally restricted	2F	Expression in kidney restricted to glomeruli and proximal tubules.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	2G	In ganglionic eminence, expression in ventricular, subventricular and mantle zones.
TS20: medulla oblongata	Present	Regionally restricted	2G	Expression in some nuclei of pons and medulla oblongata.
TS20: pons	Present	Regionally restricted	2G	Expression in some nuclei of pons and medulla oblongata.
TS20: metanephros	Present	Regionally restricted	2G	From E14.5, expression in kidney restricted to glomeruli and proximal tubules.

Structure	Level	Pattern	Image	Note
TS22: olfactory lobe	Present	Regionally restricted	2H	Expression in lateral olfactory tract.
TS22: ganglionic eminence	Present	Regionally restricted	2H	In ganglionic eminence, expression in ventricular, subventricular and mantle zones.
TS22: medulla oblongata	Present	Regionally restricted	2H	Expression in some nuclei of medulla oblongata.
TS22: cortical early proximal tubule	Present		2H	From E14.5, expression in kidney restricted to glomeruli and prximal tubules.
TS22: maturing glomerular tuft	Present		2H	From E14.5, expression in kidney restricted to glomeruli and prximal tubules.
TS22: diaphragm	Present		2H
TS22: intercostal muscle	Present		2H
TS22: rib cartilage condensation	Present		2H

Structure	Level	Pattern	Image	Note
TS24: corpus striatum	Present	Widespread	2I	Prominent expression in all layers of striatum.
TS24: diaphragm	Present		2I
TS24: intercostal muscle	Present		2I
TS24: hyoid	Strong		2I	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: limb cartilage condensation	Strong		2I	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: rib cartilage	Strong		2I	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: sensory organ cartilage	Strong		2I	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: thyroid cartilage	Strong		2I	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: vertebral cartilage condensation	Strong		2I	Strong expression in cartilage and endochondral structures, e.g. cartilage of nose and ear, thyroid and hyoid cartilage, and cartilage of ribs, vertibrae and limbs.
TS24: metanephros	Present	Regionally restricted	2I	Expression in kidney restricted to glomeruli and prximal tubules.

Structure	Level	Pattern	Image	Note
TS23: head	Present	Regionally restricted	3A	Expression in head including nasal epithelium and innervation.
TS23: olfactory epithelium	Present	Regionally restricted	3A

Structure	Level	Pattern	Image	Note
TS23: adenohypophysis	Present		3B
TS23: neurohypophysis	Present		3B

Structure	Level	Pattern	Image	Note
TS23: lens	Present		3C
TS23: retina	Present		3C
TS23: eye muscle	Present		3C
TS23: optic nerve	Present		3C

Structure	Level	Pattern	Image	Note
TS23: trigeminal nerve	Present		3D	Residual expression detectable in trigeminal nerve.

Structure	Level	Pattern	Image	Note
TS24: forelimb long bone	Strong	Regionally restricted	3E	In forelimbs, high expression in cartilaginous templates of humerus, radius, ulna, carpals and metacarpals,.
TS24: long bone epiphyseal plate proliferative zone	Present		3E	Expression in proliferating chondrocytes of growth plates.
TS24: long bone epiphyseal plate hypertrophic zone	Weak		3E	Expression fades when chondrocytes become hypertrophic.

Structure	Level	Pattern	Image	Note
TS24: cochlea epithelium	Present		3F

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Present	Regionally restricted	4B	Expression in neurons of striatum.
TS27: hindbrain	Weak	Regionally restricted	4B	Expression in hindbrain nuclei, which gradually reduced.
TS27: brain ventricular layer	Present	Widespread	4B	Expression in ventricular zone spread throughout brain.

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Present	Regionally restricted	4C	Expression in neurons of striatum.
TS27: hindbrain	Weak	Regionally restricted	4C	Expression in hindbrain nuclei, which gradually reduced.
TS27: brain ventricular layer	Present	Widespread	4C	Expression in ventricular zone spread throughout brain.

Structure	Level	Pattern	Image	Note
TS28: cerebral hemisphere white matter	Strong	Regionally restricted	4E	Intense expression in myelinated fiber tracts of cerebrum.
TS28: anterior commissure	Strong		4E
TS28: corpus callosum	Strong		4E
TS28: cerebellum white matter	Strong	Regionally restricted	4E	Intense expression in neuropil of cerebellum.

Structure	Level	Pattern	Image	Note
TS28: cerebral hemisphere white matter	Strong	Regionally restricted	4F	Intense expression in myelinated fiber tracts of cerebrum.
TS28: anterior commissure	Strong		4F
TS28: corpus callosum	Strong		4F
TS28: cerebellum white matter	Strong	Regionally restricted	4F	Intense expression in neuropil of cerebellum.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Regionally restricted	4H	Expression pattern in spinal cord most compatible with mixed glial expression.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Regionally restricted	4I	Expression pattern in spinal cord most compatible with mixed glial expression.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Strong	Regionally restricted	4J	In adult striatum, strong expression in oligodendrocytes of fiber tracts and in isolated cells with astrocytic morphology.

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Strong		4K
TS28: dentate gyrus	Present	Regionally restricted	4K	Expression in septum of dentate gyrus.
TS28: hippocampus	Present	Regionally restricted	4K	Expression in other parts of hippocampus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Strong	Regionally restricted	4L	Intense expression in neuropil of cerebellum.
TS28: cerebellum external granule cell layer	Present		4L

Structure	Level	Pattern	Image	Note
TS24: cortical early proximal tubule	Present		5A	Expression in kidney restricted to glomeruli and proximal tubules.
TS24: maturing glomerular tuft	Present		5A	Expression in kidney restricted to glomeruli and proximal tubules.
TS24: adrenal medulla	Present		5A	Expression in adrenal gland, expression restricted to medulla.
TS24: adrenal cortex	Absent		5A	Expression in adrenal gland, expression restricted to medulla.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Absent		5B	No detectable expression in adult kidney.

Structure	Level	Pattern	Image	Note
TS28: adrenal medulla	Present		5C	Expression in adrenal gland confined to medulla.
TS28: adrenal cortex	Absent		5C	Expression in adrenal gland confined to medulla.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: seminiferous tubule epithelium		Present	Regionally restricted	5D	Expression restricted to interior lining of testicular tubules, localized to Sertoli cells and not to spermatogonia.
TS28: Sertoli cell	Sertoli cell	Present		5D	Expression restricted to interior lining of testicular tubules, localized to Sertoli cells and not to spermatogonia.
TS28: spermatogonium		Absent		5D	Expression restricted to interior lining of testicular tubules, localized to Sertoli cells and not to spermatogonia.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Absent			Authors report no difference between heterozygous and homozygous mutant littermates.
TS28: adrenal medulla	Present			Authors report no difference between heterozygous and homozygous mutant littermates.
TS28: testis	Present			Authors report no difference between heterozygous and homozygous mutant littermates.

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Strong	Widespread		Significant expression throughout skeletal muscle.

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Weak			Expression faded dramatically.

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Trace		5F	Expression in only very few cells.

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Present	Regionally restricted	5G	Authors report expression in twice as many cells in homozygote as heterozygote. Selective expression in endplates of neuromuscular junctions.