Probe:	Htr1a cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: caudate-putamen	Strong		1A
TS22: globus pallidus	Strong		1A
TS22: telencephalon septum	Strong	Graded	1A	Expression is in a lateral-high to medial-low gradient.

Structure	Level	Pattern	Image	Note
TS22: caudate-putamen	Strong		1B
TS22: globus pallidus	Strong		1B
TS22: lateral preoptic area	Strong		1B
TS22: telencephalon septum	Strong		1B
TS22: hypothalamus medial zone	Present	Regionally restricted	1B	Expression is in the medial preoptic area.
TS22: hippocampus	Weak	Regionally restricted	1B

Structure	Level	Pattern	Image	Note
TS22: lateral habenular nucleus	Present		1C
TS22: medial habenular nucleus	Present		1C
TS22: ventral lateral geniculate nucleus	Present		1C
TS22: dorsal thalamus	Moderate		1C
TS22: amygdala	Weak		1C
TS22: hippocampus	Weak		1C

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present		1D
TS22: geniculate thalamic group	Present	Regionally restricted	1D	Expression is in the medial geniculate nucleus.
TS22: hypothalamus	Present	Regionally restricted	1D	Expression is in the paraventricular hypothalamic nucleus.
TS22: dorsal thalamus	Moderate		1D

Structure	Level	Pattern	Image	Note
TS24: corpus striatum	Strong		1E
TS24: caudate-putamen	Present		1E
TS24: telencephalon septum	Present		1E
TS24: olfactory cortex	Present	Regionally restricted	1E	Expression is in the olfactory tubercle.

Structure	Level	Pattern	Image	Note
TS24: caudate-putamen	Present		1F
TS24: cortical plate	Present		1F
TS24: hippocampus	Present		1F
TS24: piriform cortex	Present		1F
TS24: hypothalamus medial zone	Present	Regionally restricted	1F	Expression is in the medial preoptic area.
TS24: basal forebrain	Present	Regionally restricted	1F	Expression is in the substantia innominata.

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Strong		1G
TS24: hippocampus	Present		1G
TS24: hypothalamus	Present		1G
TS24: lateral habenular nucleus	Present		1G
TS24: medial habenular nucleus	Present		1G
TS24: reticular thalamic nucleus	Present		1G
TS24: ventral lateral geniculate nucleus	Present		1G
TS24: zona incerta	Present		1G
TS24: cortical plate	Present	Graded	1G	There is a medial high to lateral low gradient in the posterior part of the cortex.
TS24: amygdala	Weak		1G

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Strong		1H
TS24: dorsal lateral geniculate nucleus	Present		1H
TS24: hypothalamus	Present		1H
TS24: ventral lateral geniculate nucleus	Present		1H
TS24: ventral posterior lateral thalamic nucleus	Present		1H
TS24: ventral posterior medial thalamic nucleus	Present		1H
TS24: zona incerta	Present		1H
TS24: cortical plate	Present	Graded	1H	There is a medial high to lateral low gradient in the posterior part of the cortex.
TS24: amygdala	Weak		1H

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Strong		1I
TS27: caudate-putamen	Present		1I
TS27: cortical layer V	Present		1I
TS27: cortical layer VI	Present		1I
TS27: olfactory tubercle	Present		1I
TS27: telencephalon septum	Present		1I

Structure	Level	Pattern	Image	Note
TS27: caudate-putamen	Present		1J
TS27: cortical layer V	Present		1J
TS27: cortical layer VI	Present		1J
TS27: cortical plate	Present		1J
TS27: hippocampus	Present		1J
TS27: medial preoptic area	Present		1J
TS27: piriform cortex	Present		1J
TS27: basal forebrain	Present	Regionally restricted	1J	Expression is in the substantia innominata.

Structure	Level	Pattern	Image	Note
TS27: caudate-putamen	Present		1K
TS27: cingulate cortex	Present		1K
TS27: cortical layer V	Present		1K
TS27: cortical layer VI	Present		1K
TS27: hippocampus	Present		1K
TS27: piriform cortex	Present		1K
TS27: cortical plate	Present	Graded	1K	There is a caudal high to rostral low gradient.

Structure	Level	Pattern	Image	Note
TS27: dorsal thalamus	Very strong		1L
TS27: cortical layer V	Present		1L
TS27: cortical layer VI	Present		1L
TS27: cortical plate	Present		1L
TS27: hippocampus	Present		1L
TS27: lateral habenular nucleus	Present		1L
TS27: medial habenular nucleus	Present		1L
TS27: nucleus reuniens	Present		1L
TS27: ventral lateral geniculate nucleus	Present		1L
TS27: ventral posterior lateral thalamic nucleus	Present		1L
TS27: ventral posterior medial thalamic nucleus	Present		1L
TS27: amygdala	Weak		1L

Structure	Level	Pattern	Image	Note
TS24: medial habenular nucleus	Strong
TS24: reticular thalamic nucleus	Strong
TS24: ventral lateral thalamic nucleus	Strong
TS24: ventral posterior lateral thalamic nucleus	Strong
TS24: zona incerta	Strong
TS24: dorsal thalamus	Present		6A
TS24: hippocampus	Present		6A
TS24: ventral lateral geniculate nucleus	Present		6A
TS24: dorsal lateral geniculate nucleus	Weak		6A

Structure	Level	Pattern	Image	Note
TS20: brain	Absent

Structure	Level	Pattern	Image	Note
TS22: amygdala	Present
TS22: caudate-putamen	Present
TS22: cortical plate	Present
TS22: dorsal lateral geniculate nucleus	Present
TS22: globus pallidus	Present
TS22: hippocampus	Present
TS22: lateral habenular nucleus	Present
TS22: lateral preoptic area	Present
TS22: medial habenular nucleus	Present
TS22: piriform cortex	Present
TS22: reticular thalamic nucleus	Present
TS22: telencephalon septum	Present
TS22: ventral lateral geniculate nucleus	Present
TS22: ventral posterior lateral thalamic nucleus	Present
TS22: ventral posterior medial thalamic nucleus	Present
TS22: zona incerta	Present
TS22: allocortex	Present	Regionally restricted		Expression is in the dentate gyrus.
TS22: hypothalamus medial zone	Present	Regionally restricted		Expression is in the medial preoptic area.
TS22: hypothalamus	Present	Regionally restricted		Expression is in the paraventricular hypothalamic nucleus.
TS22: thalamic nucleus	Present	Regionally restricted		Includes the ventrolateral thalamic nucleus, ventromedial thalamic nucleus, and the posterior complex.

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present
TS24: caudate-putamen	Present
TS24: cortical plate	Present
TS24: dorsal lateral geniculate nucleus	Present
TS24: globus pallidus	Present
TS24: hippocampus	Present
TS24: lateral habenular nucleus	Present
TS24: medial habenular nucleus	Present
TS24: paraventricular hypothalamic nucleus	Present
TS24: paraventricular thalamic nucleus	Present
TS24: piriform cortex	Present
TS24: posterior thalamic group	Present
TS24: reticular thalamic nucleus	Present
TS24: telencephalon septum	Present
TS24: ventral lateral geniculate nucleus	Present
TS24: ventral lateral thalamic nucleus	Present
TS24: ventral posterior lateral thalamic nucleus	Present
TS24: ventral posterior medial thalamic nucleus	Present
TS24: zona incerta	Present
TS24: olfactory cortex	Present	Regionally restricted		Expression is in the olfactory tubercle.
TS24: hippocampal formation	Present	Regionally restricted		Expression is in the dentate gyrus.
TS24: thalamic nucleus	Present	Regionally restricted		Includes the ventrolateral thalamic nucleus, ventromedial thalamic nucleus, and the posterior complex.

Structure	Level	Pattern	Image	Note
TS27: amygdala	Present
TS27: caudate-putamen	Present
TS27: cortical layer V	Present
TS27: cortical layer VI	Present
TS27: cortical plate	Present
TS27: dentate gyrus	Present
TS27: dorsal lateral geniculate nucleus	Present
TS27: globus pallidus	Present
TS27: hippocampus	Present
TS27: lateral habenular nucleus	Present
TS27: medial habenular nucleus	Present
TS27: olfactory tubercle	Present
TS27: piriform cortex	Present
TS27: posterior thalamic group	Present
TS27: reticular thalamic nucleus	Present
TS27: telencephalon septum	Present
TS27: ventral lateral geniculate nucleus	Present
TS27: ventral lateral thalamic nucleus	Present
TS27: ventral posterior lateral thalamic nucleus	Present
TS27: ventral posterior medial thalamic nucleus	Present
TS27: zona incerta	Present
TS27: thalamic nucleus	Present	Regionally restricted		Expression is in the ventromedial thalamic nucleus.