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Gene Expression Data
Assay Details
Assay
Reference: J:136705 Jonckheere N, et al., Analysis of mPygo2 mutant mice suggests a requirement for mesenchymal Wnt signaling in pancreatic growth and differentiation. Dev Biol. 2008 Jun 15;318(2):224-35
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6386389
Gene symbol: Pygo2
Gene name: pygopus 2
Antibody: anti-Pygo2
Detection system: Cy3
Results
Specimen 1B/C: embryonic day 10.5 (more )
Note: Double labeled: red - Pygo2; green - Pdx1 (assay MGI:6386391).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted 1C , 1B Expression in pancreatic epithelial progenitor cells in dorsal and ventral pancreatic anlage. Level of expression varied between cells in eppithelium. Strong expression in mesenchyme surrouding both dorsal and ventral pancreatic anlage.
TS17: pancreas primordium ventral bud Present Regionally restricted 1C , 1B Expression in pancreatic epithelial progenitor cells in dorsal and ventral pancreatic anlage. Level of expression varied between cells in eppithelium. Strong expression in mesenchyme surrouding both dorsal and ventral pancreatic anlage.
Specimen 1E/F: embryonic day 12.5 (more )
Note: Double labeled: red - Pygo2; green - Pdx1 (assay MGI:6386391).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 1E , 1F Expression persists in pancreatic epithelial cells.
TS20: pancreas mesenchyme Strong Regionally restricted 1E , 1F Abundant expression in mesenchyme surrounding both dorsal and ventral pancreatic anlage.
Specimen 1H/I: embryonic day 12.5 (more )
Note: Double labeled: red - Pygo2; green - Ctnnb1 (assay MGI:6386393).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present 1H , 1I
TS20: pancreas mesenchyme Present 1H , 1I
Specimen 1K/L: embryonic day 15.5 (more )
Note: Double labeled: red - Pygo2; green - Cdh1 (assay MGI:6386394).
Structure Level Pattern Image Note
TS23: pancreas epithelium Present Widespread 1L , 1K Expression in most pancreatic epithelial cells.
TS23: pancreas mesenchyme Present Scattered 1L , 1K Scattered expression in interstitial cells.
Specimen 1N: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: pancreas epithelium Present Regionally restricted 1N Expression in same cluster of cells that expresses amylase.
Specimen 1O: embryonic day 15.5 (more )
Note: Double labeled: red - Pygo2; green - Pdx1 (assay MGI:6386391).
Structure Level Pattern Image Note
TS23: endocrine pancreas Weak Regionally restricted 1O Weak expression in newly differentiated beta cells.
TS23: pancreatic acinus Strong 1O Strong expression in epithelial cell clusters in forming exocrine acinar compartment.
Specimen 1P: embryonic day 18.5 (more )
Note: Double labeled: red - Pygo2; green - Muc1 (assay MGI:6386397).
Structure Level Pattern Image Note
TS26: pancreatic duct Present Regionally restricted 1P Expression in subset (Muc1-expressing) of ductal cells.
TS26: pancreas mesenchyme Present Scattered 1P
Specimen 1Q: embryonic day 18.5 (more )
Note: Double labeled: red - Pygo2; green - Ins1 (assay MGI:6386398) and Ins2 (assay MGI:6386399).
Structure Level Pattern Image Note
TS26: endocrine pancreas Absent 1Q No expression in insulin-expressing cells.
Specimen 1R: embryonic day 18.5 (more )
Note: Double labeled: red - Pygo2; green - Gcg (assay MGI:6386402).
Structure Level Pattern Image Note
TS26: endocrine pancreas Absent 1R No expression in glucagon-expressing cells.
TS26: pancreatic duct Present Regionally restricted 1R Expression in subset of ductal cells.
Specimen S1B/C: embryonic day 12.5 (more )
Note: Double labeled: red - Pygo2; blue - DAPI.
Structure Level Pattern Image Note
TS20: pancreas epithelium Present S1C , S1B
TS20: pancreas mesenchyme Present S1C , S1B
Specimen S1E/F: embryonic day 12.5; Pygo2tm1.1Xdai/Pygo2tm1.2Xdai, Tg(Pdx1-cre)6Cvw/0 (more )
Note: Conditional mutant. Double labeled: red - Pygo2; blue - DAPI.
Structure Level Pattern Image Note
TS20: pancreas Absent S1F , S1E Authors report complete absence of expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory