Ifit1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:265968 Mannion NM, et al., The RNA-editing enzyme ADAR1 controls innate immune responses to RNA. Cell Rep. 2014 Nov 20;9(4):1482-94
Assay type:	RT-PCR
MGI Accession ID:	MGI:6386169
Gene symbol:	Ifit1
Gene name:	interferon-induced protein with tetratricopeptide repeats 1

Probe:	Ifit1-pC, Ifit1-pD
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Values were expressed relative to wild type, which was set to one.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
wild type	E11.5	TS19: embryo	Present	Not Specified; total RNA	Not Specified		Not Specified
Adar1-/-	E11.5	TS19: embryo	Present	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6N	Adar^tm2Phs/Adar^tm2Phs	Not Specified
Adar1-/-;Ifnar1-/-	E11.5	TS19: embryo	Present	Not Specified; total RNA	involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * C57BL/6N	Adar^tm2Phs/Adar^tm2Phs, Ifnar1^tm1Agt/Ifnar1^tm1Agt	Not Specified
Adar1-/-;Mavs-/-	E11.5	TS19: embryo	Present	Not Specified; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * C57BL/6N	Adar^tm2Phs/Adar^tm2Phs, Mavs^tm1Tsc/Mavs^tm1Tsc	Not Specified

Notes:
(a) Expression was highly elevated in Adar mutant embryos compared to wild type. Levels returned to normal in Adar;Mavs double mutant embryos. The Adar;Ifnar1 double mutant did not normalize the transcript levels as fully as the Adar;Mavs mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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