Probe:	pan-Dlk1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS14: branchial arch	Present		2B
TS14: olfactory placode	Present		2B

Structure	Level	Pattern	Image	Note
TS18: 1st branchial arch mandibular component	Strong		2C
TS18: 1st branchial arch maxillary component	Strong		2C
TS18: 2nd branchial arch	Strong		2C
TS18: olfactory epithelium	Strong		2C
TS18: limb bud	Present		2C
TS18: liver	Present		2C
TS18: otocyst	Present		2C
TS18: somite	Present		2C
TS18: unsegmented mesenchyme	Present		2C

Structure	Level	Pattern	Image	Note
TS20: liver	Weak		2D
TS20: olfactory epithelium	Weak		2D
TS20: somite	Weak		2D
TS20: embryo	Weak	Regionally restricted	2D	Expression is in the branchial arch.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch mandibular component	Present		3A
TS14: olfactory placode	Present		3A

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component	Strong		3B
TS16: 1st branchial arch maxillary component	Strong		3B
TS16: olfactory epithelium	Strong		3B
TS16: 2nd branchial arch	Present		3B

Structure	Level	Pattern	Image	Note
TS18: olfactory epithelium	Present		3C	Expression is at the periphery of the olfactory epithelium and is absent in the invaginating nasal pit.
TS18: 1st branchial arch mandibular component	Present	Regionally restricted	3C
TS18: 1st branchial arch maxillary component	Present	Regionally restricted	3C
TS18: 2nd branchial arch	Present	Regionally restricted	3C

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Weak	Regionally restricted	3D	Expression is at the periphery of the olfactory epithelium.
TS20: embryo	Weak	Regionally restricted	3D	Expression is in the second branchial arch.
TS20: mandible primordium	Weak		3D
TS20: maxillary process	Present	Regionally restricted	3D

Structure	Level	Pattern	Image	Note
TS16: limb bud	Strong	Regionally restricted	4A	Expression is near the center of the developing limb and at the midline.

Structure	Level	Pattern	Image	Note
TS18: limb bud	Strong	Regionally restricted	4B	Expression is near the center of the developing limb and at the midline.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	4C	Expression is elongated along the ventro-medial axis of the limb bud.

Structure	Level	Pattern	Image	Note
TS16: unsegmented mesenchyme	Weak		4D

Structure	Level	Pattern	Image	Note
TS18: unsegmented mesenchyme	Strong	Regionally restricted	4E	Expression is as a broad band in the posterior presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS20: unsegmented mesenchyme	Present	Regionally restricted	4F	Expression is as a broad band in the posterior presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present		5A
TS18: somite	Present		5A

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present		5B
TS18: somite	Present		5B

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present		5C
TS18: somite	Present		5C

Structure	Level	Pattern	Image	Note
TS18: unsegmented mesenchyme	Present		5D