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Gene Expression Data
Assay Details
Assay
Reference: J:227506 Jimenez R, et al., Maternal SIN3A regulates reprogramming of gene expression during mouse preimplantation development. Biol Reprod. 2015 Oct;93(4):89
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6385592
Gene symbol: Sin3a
Gene name: transcriptional regulator, SIN3A (yeast)
Antibody: anti-SIN3A
Detection system: Secondary antibody coupled to Cy5
Results
Specimen 1C oocyte: postnatal week 6 (more )
Note: Germinal vesicle (GV)-intact oocytes
Structure Level Pattern Image Note
TS28: primary oocyte Present 1C Oocyte Expressed in the nucleus.
Specimen 1C MII in vivo: postnatal week 6 (more )
Note: Metaphase II oocytes
Structure Level Pattern Image Note
TS28: secondary oocyte Absent 1C MII in vivo The failure to detect the protein is attributed to the >25-fold dilution into the cytoplasm following germinal vesicle breakdown and the pronounced staining in the pronuclei due to accumulation of the newly synthesized protein.
Specimen 1C 1-cell: embryonic day 0.5 (more )
Note: 1-cell embryos
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 1C 1-cell Strongest expression in the nucleus.
Specimen 1C 2-cell: embryonic day 1.0 (more )
Note: 2-cell embryos
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 1C 2-cell Reduced expression in the nucleus compared with 1-cell embryos.
Specimen 1C 8-cell: embryonic day 2.5 (more )
Note: 8-cell embryos
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Weak 1C 8-cell Expressed in the nucleus. Lowest expression.
Specimen 1C Blastocyst: embryonic day 3.5 (more )
Note: blastocysts
Structure Level Pattern Image Note
TS4: blastocyst Present 1C Blastocyst Expressed in the nucleus.
Specimen S1 left: embryonic day 0.5 (more )
Note: 1-cell embryos, permeabilize then fix
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present S1_left Expressed in the nucleus. Similar expression between male and female pronuclei. No obvious difference for the two different protocols.
Specimen S1 right: embryonic day 0.5 (more )
Note: 1-cell embryos, fix and then permeabilize
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present S1_right Expressed in the nucleus. Similar expression between male and female pronuclei. No obvious difference for the two different protocols.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory