Gene Expression Data
Assay Details
Assay
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Reference:
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J:234935
Meng XQ, et al., Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice. J Reprod Dev. 2016 Aug 25;62(4):351-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6383976
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Gene symbol:
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Ptk2b
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Gene name:
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PTK2 protein tyrosine kinase 2 beta
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Results
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Specimen
4A2/A:
embryonic day 1.0
(more )
Note: Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
4A2/A:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 1.0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
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Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
Regionally restricted |
4A2/A
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Expression particularly in joints of the two cells and this nuclei, but not in nucleoli.
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Specimen
4B2/B:
embryonic day 2.0
(more )
Note: Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
4B2/B:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 2.0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 4-cell stage embryo |
Present |
Regionally restricted |
4B2/B
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Expression increasingly weakened until the morula stage. Expression throughout cytoplasm. Punctate distribution of expression, presented as many points or peaches.
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Specimen
4C2/C:
embryonic day 2.5
(more )
Note: Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
4C2/C:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS3: 8-cell stage embryo |
Present |
Regionally restricted |
4C2/C
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Expression increasingly weakened until the morula stage. Expression throughout cytoplasm. Punctate distribution of expression, presented as many points or peaches.
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Specimen
4D2/D:
embryonic day 3.0
(more )
Note: Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
4D2/D:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 3.0
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Regionally restricted |
4D2/D
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Expression increasingly weakened until the morula stage. Expression in the perinuclear region.
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Specimen
4E2/E:
embryonic day 3.5
(more )
Note: Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
Specimen
4E2/E:
(close )
Genetic Background:
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Kunming
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - phosphorylated Ptk2b; red - propidium iodide (PI, for DNA).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
Regionally restricted |
4E2/E
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Expression in perinuclear region and joint of cells. No expression in nuclei.
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