Bap1 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:279204 Ferrando RE, et al., Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice. J Histochem Cytochem. 2015 Apr;63(4):244-55
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6380650
Gene symbol:	Bap1
Gene name:	Brca1 associated protein 1

Reporter:	FLAG
Antibody:	Anti-Flag tag antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	A weak background signal was also seen in control wild-type tissues.

Results

Specimen 8A: postnatal week 14; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Pattern	Image	Note
TS28: bronchiole epithelium	Present		8A
TS28: lung alveolus	Present		8A	Nuclear expression in the type II pneumocytes. Weaker than that in the bronchiolar epithelium.

Specimen 8B: postnatal week 14; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Pattern	Image	Note
TS28: liver	Present		8B	Nuclear expression in the Kupffer cells.

Specimen 8C: postnatal week 14; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Pattern	Image	Note
TS28: lymph node	Present		8C	Nuclear expression in the lymphocytes.

Specimen 8G: postnatal week 14; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Pattern	Image	Note
TS28: bronchiole epithelium	Present		8G
TS28: lung alveolus	Present		8G	Nuclear expression in the type II pneumocytes. Weaker than that in the bronchiolar epithelium.

Specimen 8H: postnatal week 14; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Pattern	Image	Note
TS28: liver	Present		8H	Nuclear expression in the Kupffer cells.

Specimen 8I: postnatal week 14; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Pattern	Image	Note
TS28: lymph node	Present		8I	Nuclear expression in the lymphocytes.

Specimen 9 BAP1.3xFLAG: postnatal adult; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Pattern	Image	Note
TS28: spleen	Present		9_right lower left

Specimen not shown: postnatal week 14; Bap1^tm1.1Vmd/Bap1^tm1.1Vmd (more

)

Structure	Level	Note
TS28: gallbladder epithelium	Present
TS28: spleen	Present	Nuclear expression in the lymphocytes.
TS28: thymus	Present	Nuclear expression in the lymphocytes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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