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Gene Expression Data
Assay Details
Assay
Reference: J:279204 Ferrando RE, et al., Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice. J Histochem Cytochem. 2015 Apr;63(4):244-55
Assay type: Western blot
MGI Accession ID: MGI:6380545
Gene symbol: Ripk3
Gene name: receptor-interacting serine-threonine kinase 3
Antibody: anti-RIPK3 (1G6)
Detection system: Secondary antibody
Results Image: 4_RIPK3
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Size Not Specified (c) Genetic BackgroundMutant Allele(s)Sex
Bone Marrow +/+ P TS28: bone marrow Absent Absent Absent Not Specified Not Specified
Bone Marrow FLAG/FLAG P TS28: bone marrow Absent Absent Absent involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Brain +/+ P TS28: brain Absent Absent Absent Not Specified Not Specified
Brain FLAG/FLAG P TS28: brain Absent Absent Absent involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Heart +/+ P TS28: heart Absent Absent Absent Not Specified Not Specified
Heart FLAG/FLAG P TS28: heart Absent Absent Absent (d) involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Kidney +/+ P TS28: metanephros Absent Absent Absent Not Specified Not Specified
Kidney FLAG/FLAG P TS28: metanephros Absent Absent Absent involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Liver +/+ P TS28: liver Absent Absent Absent Not Specified Not Specified
Liver FLAG/FLAG P TS28: liver Absent Absent Absent (d) involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Lung +/+ P TS28: lung Absent Absent Absent Not Specified Not Specified
Lung FLAG/FLAG P TS28: lung Absent Absent Weak involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Spleen +/+ P TS28: spleen Present Weak Absent Not Specified Not Specified
Spleen FLAG/FLAG P TS28: spleen Present Absent Present involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Thymus +/+ P TS28: thymus Weak Present Absent Not Specified Not Specified
Thymus FLAG/FLAG P TS28: thymus Weak Absent Present involves: C57BL/6 Ripk3tm2.1Geno/Ripk3tm2.1GenoNot Specified
Notes:
(a) Additional lower molecular weight band, which is suspected to be a cleavage product or splice variant.
(b) RIPK3
(c) RIPK3.3xFLAG
(d) When used anti-FLAG antibody, a band was detected, suggesting that the 3FLAG tag can improve assay sensitivity.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory