Gene Expression Data
Assay Details
Assay
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Reference:
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J:34321
White FA, et al., Synchronous onset of NGF and TrkA survival dependence in developing dorsal root ganglia. J Neurosci. 1996 Aug 1;16(15):4662-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6379760
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Gene symbol:
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Ngf
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Gene name:
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nerve growth factor
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Probe:
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SD39-NGF
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Probe preparation:
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Antisense labelled with P33 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3 E11.5:
embryonic day 11.5
(more )
Specimen
3 E11.5:
(close )
Genetic Background:
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either: 129S2/SvPas or CF-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3 E13.5:
embryonic day 13.5
(more )
Specimen
3 E13.5:
(close )
Genetic Background:
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either: 129S2/SvPas or CF-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3 E15.5:
embryonic day 15.5
(more )
Specimen
3 E15.5:
(close )
Genetic Background:
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either: 129S2/SvPas or CF-1
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: hindlimb skin |
Very strong |
Regionally restricted |
3_E15.5 NGF
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Expression is absent in the most distal part of the hindlimb.
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TS23: hindlimb mesenchyme |
Very strong |
Regionally restricted |
3_E15.5 NGF
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Expression is in the mesenchyme subjacent to the epithelium and is absent in the most distal part.
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Specimen
not shown E10.5:
embryonic day 10.5
(more )
Specimen
not shown E10.5:
(close )
Genetic Background:
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either: 129S2/SvPas or CF-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown E15.5:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntrk1tm1Bbd/Ntrk1tm1Bbd
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: hindlimb |
Present |
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Expression in this mutant is the same as in wild-type embryos.
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