Wee1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:171197 Oh JS, et al., Protein tyrosine kinase Wee1B is essential for metaphase II exit in mouse oocytes. Science. 2011 Apr 22;332(6028):462-5
Assay type:	RT-PCR
MGI Accession ID:	MGI:6369821
Gene symbol:	Wee1
Gene name:	WEE 1 homolog 1 (S. pombe)

Probe:

Wee1A-pA, Wee1A-pB

Results

Image: 1A Wee1A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
-	Control
GV	P adult	TS28: primary oocyte	Absent	Not Specified; total RNA	C57BL/6		Female
MII	P adult (a)	TS28: secondary oocyte	Absent	Not Specified; total RNA	C57BL/6		Female
1-cell	E0.5 (a)	TS1: 1-cell stage embryo	Absent	Not Specified; total RNA	C57BL/6		Not Specified
2-cell	E1.0 (a)	TS2: 2-cell stage embryo	Ambiguous (b)	Not Specified; total RNA	C57BL/6		Not Specified
4-cell	E2.0 (a)	TS3: 4-cell stage embryo	Ambiguous (b)	Not Specified; total RNA	C57BL/6		Not Specified
8-cell	E2.5 (a)	TS3: 8-cell stage embryo	Present	Not Specified; total RNA	C57BL/6		Not Specified
blastocyst	E3.5 (a)	TS4: blastocyst	Present	Not Specified; total RNA	C57BL/6		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Authors report Wee1B (Wee2) was replaced by Wee1A (Wee1) during the 2-cell or 4-cell embryo mitotic divisions.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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