Hsf2 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74044 Eriksson M, et al., Heat shock factor 2 is activated during mouse heart development. Int J Dev Biol. 2000 Aug;44(5):471-7
Assay type:	Western blot
MGI Accession ID:	MGI:6369647
Gene symbol:	Hsf2
Gene name:	heat shock factor 2

Antibody:	anti-HSF2
Detection system:	horseradish peroxidase

Results

Image: 3A HSF2

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Genetic Background	Mutant Allele(s)	Sex
F8.5	E8.5	TS13: embryo	Ambiguous	Trace	Weak	NMRI		Not Specified
10.5	E10.5	TS17: heart	Ambiguous	Trace	Weak	NMRI		Not Specified
11.5	E11.5	TS19: heart	Present	Present	Strong (d)	NMRI		Not Specified
12.5	E12.5	TS20: heart	Present	Present	Strong (e)	NMRI		Not Specified
13.5	E13.5	TS21: heart	Ambiguous	Weak	Present	NMRI		Not Specified
14.5	E14.5	TS22: heart	Ambiguous	Weak	Present	NMRI		Not Specified
16.5	E16.5	TS24: heart	Ambiguous	Weak	Present	NMRI		Not Specified
18.5	E18.5	TS26: heart	Ambiguous	Weak	Present	NMRI		Not Specified
HS	Treated sample: no data stored
PC12 0	Cultured cells: no data stored
PC12 HS	Cultured cells: no data stored

Notes:
(a) Higher molecular weight band transiently upregulated at stages E11.5 and E12.5..
(b) Alpha isoform.
(c) Beta isoform was more abundant than alpha isoform.
(d) Expression levels 8-fold compared to that seen at E18.5
(e) Expression levels 6-fold compared to that seen at E18.5

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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